贵州农业科学2026，Vol.54Issue(3)：22-33,12.DOI:10.3969/j.issn.1001-3601.2026.03.003
贵州香蕉炭疽病病原菌的分离鉴定与防治药剂筛选
Isolation and Identification of Banana Anthracnose Pathogens and Screening of Fungicides Against Anthracnose in Guizhou
摘要
Abstract
[Objective]The species of pathogenic strains causing banana anthracnose were investigated to screen effective chemical fungicides for controlling banana anthracnose and realize scientific and precise application of fungicides for guaranteeing economic benefits and sustainable development of banana industry.[Method]The morphological characteristics,pathogenicity and species of the purified stain causing banana anthracnose isolated from the suspected banana anthracnose leaves collected from four banana producing areas(Ceheng County,Wangmo County,Anlong County and Xingyi City)in Guizhou were analyzed,verified and identified by observation,Koch's law and molecular biology respectively.The indoor toxicity and controlling efficacy of 50%carbendazim WP,25%myclobutanil EC and 75%trifloxystrobin •tebuconazole WDG against the purified stain causing banana anthracnose were detected by the mycelial growth rate method.[Result]The strain GZXY012 was successfully isolated from the suspected banana anthracnose leaves and identified as Colletotrichum fructicola by morphology and molecular biology.There was a significant difference in inhibitory effect against C.fructicola among 8 tested fungicides,including 50%carbendazim WP,75%trifloxystrobin•tebuconazole WDG,25%myclobutanil EC and 80%mancozeb WP,etc.The EC50 of 45%prochloraz EW,50%carbendazim WP and 70%thiophanate-methyl WP was 0.095 μg/mL,0.176 μg/mL and 1.343 μg/mL respectively.The EC50 of 25%pyraclostrobine SC,10%difenoconazole M and 75%trifloxystrobin •tebuconazole WDG was 4.648-6.416 μg/mL.The EC50 of 25%Myclobutanil EC and 80%Mancozeb WP was 24.713 μg/mL and 91.332 μg/mL.The control effect of 6 tested fungicides×500 times against C.fructicola was 45%prochloraz EW(94.44%)＞50%carbendazim WP(92.22%)=10%difenoconazole M(92.22%)＞75%trifloxystrobin •tebuconazole WDG(88.89%)＞70%thiophanate-methyl WP(86.66%)＞25%pyraclostrobine SC(85.56%).[Conclusion]C.fructicola is one of main pathogens causing anthracnose in Guizhou's banana-growing regions.Three fungicides of prochloraz,carbendazim and difenoconazole with a good control effect against C.fructicola causing banana anthracnose should be used in banana production in Guizhou.关键词
香蕉/炭疽病/分离纯化/形态学特征/分子生物学鉴定/病原鉴定/杀菌剂/防治效果Key words
banana/anthracnose/isolation and purification/morphological characteristics/molecular biological identification/pathogen identification/fungicide/control effect分类
农业科技引用本文复制引用
熊新颖,刘凡值,韩树全,刘荣,张剑,魏鹏程,贺尔奇..贵州香蕉炭疽病病原菌的分离鉴定与防治药剂筛选[J].贵州农业科学,2026,54(3):22-33,12.基金项目
贵州省农业科学院一般基金项目"'金粉1号'品质形成的主要影响因子的研究"(黔农科一般基金[2025]54),"香蕉杂交后代的倍性鉴定及优株筛选"(黔农科一般基金[2025]41),"干旱胁迫对苗药芭蕉根生长及药用成分形成机制的研究"(黔农科一般基金[2024]10) （黔农科一般基金[2025]54）
贵州省农业科学院青年基金项目"贵州香蕉主要病害种类鉴定及其发生分布规律研究"(黔农科青年基金[2023]26) （黔农科青年基金[2023]26）
国家香蕉产业技术体系贵州黔西南综合试验站(CARS-31-23) （CARS-31-23）
国家重点研发计划项目"册亨县香蕉产业提质增效关键技术集成与示范"(2022YFD1601706) （2022YFD1601706）
贵州省农业科学院种质资源项目"贵州热区特色作物种质创新与高效关键技术研究"黔农科种质资源([2023]008号) （[2023]008号）
