高等学校化学学报2026，Vol.47Issue(4)：102-110,9.DOI:10.7503/cjcu20250410
具有聚集诱导发光性质的高灵敏度和高光稳定性的脂滴荧光探针
Novel AIE Fluorescent Probes for Ultrahigh Sensitivity and High Photostability in Lipid Droplets Imaging
摘要
Abstract
Two novel aggregation-induced emission(AIE)-active probes,TPA-H and TPA-2F,were designed and synthesized based on a triphenylamine(TPA)core.Systematic characterization demonstrated that both probes exhibit excellent biocompatibility(cell viability>90%at concentrations up to 50 µmol/L)and outstanding LD-targeting speci-ficity with minimal colocalization with other organelles such as mitochondria and lysosomes.During early differentia-tion of 3T3-L1 adipocytes,both TPA-2F and TPA-H clearly visualized small and nascent LDs that were difficult to be detected with BODIPY,indicating superior imaging sensitivity compared to the existing fluorescent probes for LDs.Moreover,TPA-2F demonstrated exceptional photostability,retaining over 90%of its initial fluorescence intensity after 100 continuous laser scanning cycles,significantly outperforming TPA-H.This work not only provides two high-performance LD imaging tools but also highlights the potential of AIE luminogens(AIEgens)in organelle-specific bioimaging,offering promising avenues for early diagnosis and mechanistic research of lipid-related metabolic diseases.关键词
聚集诱导发光(AIE)/荧光成像/脂滴/光稳定性Key words
Aggregation-induced emission(AIE)/Fluorescence imaging/Lipid Droplets/Photostability分类
化学化工引用本文复制引用
高鑫,卿佳,胡祎辰,上官之春,梁同玲,周永胜,张关心,张德清..具有聚集诱导发光性质的高灵敏度和高光稳定性的脂滴荧光探针[J].高等学校化学学报,2026,47(4):102-110,9.基金项目
国家自然科学基金(批准号:82530030,82270954,22021002)资助. Supported by the National Natural Science Foundation of China(Nos.82530030,82270954,22021002). （批准号:82530030,82270954,22021002）
