河北医学2026，Vol.32Issue(3)：382-389,8.DOI:10.3969/j.issn.1006-6233.2026.03.05
鲁斯可皂苷元调节SIRT3/PPARγ信号通路对急性心肌梗死后心力衰竭小鼠的心肌保护作用
The Cardioprotective Effect of Ruscogenin on Mice with Heart Failure after Acute Myocardial Infarction by Regulating the SIRT3/PPARγ Signaling Pathway
摘要
Abstract
Objective:To explore the cardioprotective effect of ruscogenin(RUS)on mice with heart failure(HF)after acute myocardial infarction by regulating the silent information regulator 3(SIRT3)/peroxi-some proliferator-activated receptor gamma(PPAR gamma)signaling pathway.Methods:A mouse model of HF was established after acute myocardial infarction,and successfully constructed mice were randomly separa-ted into HF group,RUS low-dose group,RUS high-dose group,and 3-TYP group(RUS high-dose+SIRT3/PPARγ signaling pathway inhibitor 3-TYP).Another 10 mice were randomly included as the Con group.Mouse heart function related indicators were detected.The myocardial markers,inflammatory and oxidative stress-related factors in mouse serum were detected.Pathological and fibrotic changes in mouse myocardial tissue were observed.The apoptosis rate of mouse myocardial cells was detected.In addition,Western blot was used to measure the SIRT3/PPARγ signaling pathway and apoptosis related protein expression in myocar-dial tissue.Results:Compared with the Con group,the HF group showed severe pathological damage and fi-brosis in mouse myocardial tissue,with a large amount of inflammatory cell infiltration,myocardial cell necro-sis,decreased left ventricular ejection fraction(LVEF),left ventricular fractional shortening(LVFS),SOD,Bcl-2,SIRT3,and PPARγ,and raised left ventricular end diastolic volume(LVEDV),left ventricular end systolic volume(LVESV),creatine kinase isoenzyme(CK-MB),lactate dehydrogenase(LDH),cardiac troponin T(cTnT),TNF-α,IL-6,IL-8,MDA,ROS,myocardial cell apoptosis rate,and Bax(P＜0.05).Compared with the HF group,the low-dose RUS group and high-dose RUS group showed improved pathologi-cal damage and fibrosis of mouse myocardial tissue,reduced inflammatory cell infiltration and myocardial cell necrosis,raised LVEF,LVFS,SOD,Bcl-2,SIRT3,PPARγ,and decreased LVEDV,LVESV,CK-MB,LDH,cTnT,TNF-α,IL-6,IL-8,MDA,ROS,myocardial cell apoptosis rate,and Bax(P＜0.05).Com-pared with the high-dose RUS group,the 3-TYP group showed aggravated pathological damage and fibrosis in mouse myocardial tissue,raised inflammatory cell infiltration and myocardial cell necrosis,prominently de-creased LVEF,LVFS,SOD,Bcl-2,SIRT3,PPARγ,and prominently raised LVEDV,LVESV,CK-MB,LDH,cTnT,TNF-α,IL-6,IL-8,MDA,ROS,myocardial cell apoptosis rate,and Bax(P＜0.05).Con-clusion:RUS may have a protective effect on myocardium of mice with HF after acute myocardial infarction by activating the SIRT3/PPARγ signaling pathway.关键词
急性心肌梗死后心力衰竭/鲁斯可皂苷元/沉默信息调节因子3/过氧化物酶体增殖物激活受体γ/心肌保护Key words
Heart failure after acute myocardial infarction/Ruscogenin/Silent information regu-lator 3/peroxisome proliferator-activated receptor gamma/Myocardial protection引用本文复制引用
路陆,曹程,庞文一,赵阳,卢苓苓..鲁斯可皂苷元调节SIRT3/PPARγ信号通路对急性心肌梗死后心力衰竭小鼠的心肌保护作用[J].河北医学,2026,32(3):382-389,8.基金项目
河北省2025年度医学科学研究课题计划,(编号:20251568) （编号:20251568）
