河北医学2026，Vol.32Issue(3)：403-408,6.DOI:10.3969/j.issn.1006-6233.2026.03.08
五味子乙素抑制CCL2-CCR2信号轴减轻咳嗽变异型哮喘大鼠肺损伤
Schisandrin B Alleviates Lung Injury in Asthma Rats with Variant Cough Through Inhibiting CCL2-CCR2 Signaling Axis
摘要
Abstract
Objective:To investigate the effects of schisandrin(SchB)on cough variant asthma(CVA)rats and CC chemokine ligand 2(CCL2)/CC-like chemokine receptor 2(CCR2)signaling axis.Methods:The CVA rat model was established,and the rats were divided into Control group,model group,Schisandra B low,medium and high dose groups(SchB-L,SchB-M and SchB-H groups)and schisandra B high dose+CCL2 recombinant protein(SchB-H+CCL2 group).The cough frequency,forced expiratory volume(FEV1),FEV1/forced vital capacity(FVC)(FEV1/FVC)ratio,the levels of interleukin-4(IL-4),in-terleukin-5(IL-5),interferon-γ(IFN-γ)and leutriene C4(LTC4)as well as eosinophilic count(EOS)in alveolar lavage fluid were detected.The pathological changes of lung tissue were observed.The expression levels of osteopontin(OPN),CCL2 and CCR2 in lung tissue were detected.Results:In CVA group,lung tissue injury was serious,cough frequency was increased,expressions of IL-4,IL-5,EOS,LTC4,OPN,CCL2,CCR2 were increased,and levels of FEV1,FEV1/FVC,PEF,IFN-γ were decreased(P＜0.05);SchB could alleviate lung tissue injury,reduce the number of cough,decrease the expression of IL-4,IL-5,EOS,LTC4,OPN,CCL2,CCR2,and increase the levels of FEV1,FEV1/FVC,PEF,IFN-γ(P＜0.05);CCL2 recombinant protein could partially reverse the therapeutic effect of Sch B on CVA rats(P＜0.05).Conclusion:Schisandrin B may exert therapeutic effects on CVA rats by inhibiting the CCL2-CCR2 signaling axis.关键词
咳嗽变异型哮喘/五味子乙素/CC趋化因子配体2/CC类趋化因子受体2Key words
Cough variant asthma/Schisandrin B/CC chemokine ligand 2/CC chemokine ligand receptor 2引用本文复制引用
王华乔,明绘,肖晨汐,李亚时..五味子乙素抑制CCL2-CCR2信号轴减轻咳嗽变异型哮喘大鼠肺损伤[J].河北医学,2026,32(3):403-408,6.基金项目
武汉市卫生健康委科研项目,(编号:WZ19C34) （编号:WZ19C34）
