海南师范大学学报（自然科学版）2026，Vol.39Issue(1)：57-64,8.DOI:10.12051/j.issn.1674-4942.2026.01.008
枳实水提物的降脂活性
Hypolipidemic Activity of the Water Extract of Aurantii Fructus Immaturus
摘要
Abstract
To investigate the lipid-lowering effects and oxidative stress modulation mechanisms of Aurantii Fructus Immaturus water extract(AFIWE)on a cellular model of non-alcoholic fatty liver disease(NAFLD),this study obtained AFIWE through water reflux extraction.The cytotoxicity of AFIWE on HepG2 cells was assessed using the CCK-8 assay.For the lipid-lowering experiment,an NAFLD model was established by free fatty acid(FFA)induction,with experimental groups including a blank control group,model group,AFIWE intervention groups(25,50 and 100 µg/mL),and a positive control group(25 µg/mL silymarin).Intracellular triglyceride(TG)and total cholesterol(TC)levels were measured using commercial assay kits.Lipid droplet accumulation was observed via Oil Red O staining,and the activities of superoxide dismutase(SOD),catalase(CAT),glutathione(GSH),as well as the levels of malondialdehyde(MDA)and reactive oxygen species(ROS),were determined.Results demonstrated that AFIWE at concentrations ranging from 25 to 400 µg/mL exhibited no significant cytotoxicity(cell viability>94%).Compared with the model group,AFIWE at 50 and 100 µg/mL reduced intracellular TG content by 28.6%and 41.2%,and TC content by 25.3%and 38.7%,respectively.Lipid droplet accumulation was also reduced in a concentration-dependent manner,with the 100 µg/mL group showing lipid-lowering effects comparable to those of silymarin(p>0.05).Furthermore,AFIWE at 50 and 100 µg/mL significantly enhanced SOD activity by 21.5%and 32.8%,increased CAT activity by 18.7%and 29.4%,elevated GSH content by 23.1%and 35.6%,reduced MDA levels by 26.8%and 40.3%,and decreased ROS levels by 30.2%and 42.5%,respectively.In conclusion,AFIWE exerted lipid-lowering effects by regulating oxidative stress and lipid metabolism,providing experimental evidence for the prevention and treatment of NAFLD.关键词
枳实水提物/非酒精性脂肪肝(NAFLD)/HepG2细胞/降脂活性Key words
water extract of Aurantii Fructus Immaturus/non-alcoholic fatty liver disease(NAFLD)/HepG2 cells/hypolipidemic activity分类
医药卫生引用本文复制引用
王潇,许昕妍,谌燕,陈荫..枳实水提物的降脂活性[J].海南师范大学学报（自然科学版）,2026,39(1):57-64,8.基金项目
浙江省自然科学基金项目(LQ25H30045) （LQ25H30045）
浙江省新苗人才计划项目(24127000107) （24127000107）
