海南师范大学学报（自然科学版）2026，Vol.39Issue(1)：65-71,7.DOI:10.12051/j.issn.1674-4942.2026.01.009
黄精提取物对α-葡萄糖苷酶的抑制作用
α-Glucosidase Inhibitory Activity of the Extracts from Polygonatum sibiricum
摘要
Abstract
To explore the α-glucosidase inhibitory active substances in Polygonatum sibiricum and clarify their mechanism of action,two extraction methods,water extraction and 80%ethanol extraction,were adopted to extract the same mass of Polygonatum sibiricum powder.After vacuum concentration and freeze-drying,dry products of water extract and ethanol extract were obtained.The contents of main bioactive substances including polysaccharides,polyphenols and flavonoids in the two extracts were determined.Combined with the determination of α-glucosidase inhibitory activity,the correlation between active substance contents and enzyme inhibition rate was analyzed.The extract with stronger inhibitory effect was subjected to enzymatic kinetic analysis to clarify its inhibitory type.The results showed that the yield of water extract(63.56%)was higher than that of ethanol extract(56.72%).The polysaccharide content in water extract(74.92%)was significantly higher than that in ethanol extract(48.80%),while the contents of polyphenols(0.510%)and flavonoids(0.021%)in ethanol extract were higher than those in water extract(0.380%and 0.003%,respectively).The ethanol extract exhibited stronger α-glucosidase inhibitory activity than the water extract,but weaker than the positive control acarbose.Enzyme kinetic analysis indicated that its inhibition type was uncompetitive inhibition.This study provides theoretical basis and experimental support for the screening and development of α-glucosidase inhibitory active substances in Polygonatum sibiricum.关键词
黄精提取物/生物活性物质/α-葡萄糖苷酶/抑制作用/酶促动力学Key words
Polygonatum sibiricum extract/bioactive substance/α-glucosidase/inhibitory effect/enzyme kinetics分类
轻工纺织引用本文复制引用
何晓梅,赵文慧,杨康龙,余荧,高影..黄精提取物对α-葡萄糖苷酶的抑制作用[J].海南师范大学学报（自然科学版）,2026,39(1):65-71,7.基金项目
安徽省高校自然科学研究重点项目(2024AH051992) （2024AH051992）
国家级大学生创新训练项目(202410376009) （202410376009）
安徽省大学生创新训练项目(S202310376057) （S202310376057）
