河北医学2026，Vol.32Issue(3)：436-443,8.DOI:10.3969/j.issn.1006-6233.2026.03.013
资癸益冲方通过VEGF/Notch1信号调节卵巢血管生成和自噬改善大鼠卵巢储备功能下降
Zigui Yichong Formula Improves Diminished Ovarian Reserve in Rats by Regulating Ovarian Angiogenesis and Autophagy Through VEGF/Notch1 Signaling
摘要
Abstract
Objective:To investigate the mechanism of Zigui Yichong Formula on ovarian angiogenesis in rats with diminished ovarian reserve,and analyze its relationship with VEGF/Notch1 signaling.Methods:Forty SPF-grade female SD rats were selected as experimental animals.33 rats with regular estrous cycles were determined by vaginal exfoliated cell smears and randomly divided into blank control group,model group,Zigui Yichong formula group and Zigui Yichong formula+Axitinib group,with 8 rats in each group.The ovarian wet weight,ovarian index,morphological observation,sex hormones,number of follicles,Ovarian angiogenesis,autophagy and related protein expression,as well as VEGF/Notch1 signal expression of each group were com-pared.Results:Compared with the blank control group,primary follicles,AMH,sinus follicles,VEGF,o-varian wet weight,E2,ovarian index,primordial follicles,and CD31 were decreased,and Lc3 Ⅱ,FSH,atretic follicles,Beclin1,LH,Notch1,and autophagy lysosome number levels were increased in the model and ZiqiYiFong Formula+Axitinib groups.Compared with the model group and Zigui Yichong Formul+Ax-itinib group,in the Zigui Yichong Formula group,CD31,primary follicle,VEGF,ovarian index,E2,primor-dial follicle,ovarian wet weight,sinusoidal follicle,and AMH levels were increased,and the levels of Bec-lin1,LH,Lc3 Ⅱ,atretic follicle,Notch 1,autophagy lysosome number,and FSH levels were decreased,there was statistically significant differences(P＜0.05).Conclusion:Zigui Yichong Formula can improve ovarian reserve in DOR rats through multiple pathways,including regulating sex hormones,promoting ovarian angio-genesis,and reducing autophagy,by modulating the VEGF/Notch1 signaling pathway.关键词
卵巢储备功能下降/资癸益冲方/血管内皮生长因子/跨膜体受体蛋白信号/卵巢血管生成Key words
Decreased ovarian reserve function/Zigui Yichong formula/Vascular endothelial growth factor/transmembrane body receptor protein signaling/Ovarian angiogenesis引用本文复制引用
魏学聪,赵胜男,高靖,乔宇,张雨露,常秀峰..资癸益冲方通过VEGF/Notch1信号调节卵巢血管生成和自噬改善大鼠卵巢储备功能下降[J].河北医学,2026,32(3):436-443,8.基金项目
2023年度中医药类科学研究课题计划项目,(编号:2023185) （编号:2023185）
