河北医学2026，Vol.32Issue(3)：444-450,7.DOI:10.3969/j.issn.1006-6233.2026.03.014
七氟烷麻醉对青光眼大鼠视网膜神经节细胞凋亡和视网膜影响的实验研究
Experimental Study on the Effects of Sevoflurane Anaesthesia on Retinal Ganglion Cell Apoptosis and Retinal Function in Glaucoma Rats
摘要
Abstract
Objective:To investigate the effects of sevoflurane anesthesia on the apoptosis of retinal gan-glion cells and the retina in glaucoma rats.Methods:Twelve rats were randomly seperated from forty to make a sham operation group.The other 28 rats were used to establish glaucoma rat models,among which 4 failed.The remaining 24 rats were randomly divided into a model group of 12 rats and a sevoflurane group of 12 rats.The intraocular pressure changes,pathological changes,retinal thickness,ganglion number,oxidative stress indicators,retinal morphological cell density,mitochondrial damage,the number of retinal ganglion cell apop-tosis and endoplasmic reticulum stress pathways in each group(at different times)were compared.Results:After modeling,the intraocular pressure in the three groups of rats showed a gradually increasing trend over time.Compared with the sham operation group,the intraocular pressure in the model group and the sevoflu-rane group(1 day,2 days,4 days,and 7 days after modeling)increased,and the difference was statistical-ly significant(P＜0.05).Compared with the sham operation group,in the sevoflurane group and the model group,the levels of the outer nuclear layer,Cytc/a,inner plexiform layer,P/O,retinal thickness,H2O2,outer plexiform layer,ganglion number,and ganglion cell layer decreased,while the levels of ROS,apoptotic cells,SOD,GRP78,MDA,and CHOP increased.Compared with the model group,the levels of P/O,outer plexiform layer,Cytc/a,ganglion cell layer,H2O2,ganglion number,inner plexiform layer,retinal thick-ness,and outer nuclear layer in the sevoflurane group increased,while the levels of MDA,apoptotic cells,SOD,GRP78,ROS,and CHOP decreased.The differences were statistically significant(P＜0.05).Con-clusion:Sevoflurane anesthesia can reduce the apoptosis of retinal ganglion cells in glaucoma rats,improve oxidative stress,mitochondrial damage and endoplasmic reticulum stress,increase the number of ganglia,en-hance retinal thickness and the density of retinal morphological cells,and thereby exert a protective effect on the retinal nerve fiber layer.关键词
视网膜神经节细胞凋亡/七氟烷/青光眼/视网膜/线粒体受损Key words
Retinal ganglion cell apoptosis/Sevoflurane/Glaucoma/Retina/Mitochondrial damage引用本文复制引用
马丽华,唐广贤,李凡,唐稳稳,王珊珊,王婷..七氟烷麻醉对青光眼大鼠视网膜神经节细胞凋亡和视网膜影响的实验研究[J].河北医学,2026,32(3):444-450,7.基金项目
2020年度医学科研课题,(编号:20201420) （编号:20201420）
