环境工程学报2026，Vol.20Issue(3)：752-762,11.DOI:10.12030/j.cjee.202507111
脉冲电场强化厌氧氨氧化颗粒污泥脱氮性能及微生物菌群结构响应
Enhancing nitrogen removal of anammox granular sludge with pulsed electric field and the response of microbial community structure
摘要
Abstract
This study addresses the challenges of slow growth of anaerobic ammonium oxidation(anammox)bacteria,their susceptibility to inhibition under high nitrogen loads,and reduced microbial activity.A circular pulsed electric field(1.5 V voltage,4 h electrification duration)was introduced as a regulatory strategy to systematically explore its optimization mechanisms on the nitrogen removal performance of anammox granular sludge and the associated microbial communities.By comparing the operational effects of two anaerobic sequencing batch reactors(R1:no electric field;R2:electric field applied)under varying influent nitrogen loads,the results revealed that when at loads below 1 155 mg·(L·d)-1,the total nitrogen removal efficiency of R2 was 7.5%～17.0%higher than that of R1.The biomass,specific anammox activity,and extracellular polymeric substance(EPS)secretion in R2 were also enhanced by 5%～7%,21%～71%,and 54%～77%,respectively,indicating that the external electric field strengthened the system by promoting metabolism of functional bacteria and EPS secretion.However,when the nitrogen load exceeded 1 320 mg·(L·d)-1,inhibition from NO2--N toxicity became dominant,and the enhancing effect of electric field weakened or even reversed.Microbial community analysis demonstrated that under moderate-to-low loads,the relative abundances of Planctomycetes phylum and anammox bacteria(Candidatus Brocadia and Candidatus Jettenia)in R2 were higher than those in R1,alongside elevated microbial richness(Chao1 index)and diversity(Shannon/Simpson indices).Conversely,under high nitrogen loads,microbial richness in R2 decreased below that of R1.Principal component analysis(PCA)and redundancy analysis(RDA)further confirmed that the pulsed electric field was the key driver of microbial community divergence under moderate-to-low loads,while the NO2--N concentration became the dominant regulatory parameter under high loads.This study provides theoretical insights for enhancing the shock-load resistance and promoting the engineering application of the anammox process关键词
脉冲电场/颗粒污泥/厌氧氨氧化/微生物群落Key words
pulsed electric field/granular sludge/anammox/microbial community分类
资源环境引用本文复制引用
王旭中,张宁,张鹏宇,苗娟,张瑞昌,周鸣,魏学锋..脉冲电场强化厌氧氨氧化颗粒污泥脱氮性能及微生物菌群结构响应[J].环境工程学报,2026,20(3):752-762,11.基金项目
河南省科技攻关项目(242102321064) （242102321064）
