黑龙江畜牧兽医Issue(4)：155-162,8.DOI:10.13881/j.cnki.hljxmsy.2025.04.0007
基于垂体转录组测序技术的北极狐体型变异相关基因筛选
Screening of genes related to body size variation in Alopex lagopus based on pituitary transcriptome sequencing technology
摘要
Abstract
In order to screen the genes related to body size variation in Alopex lagopus,three individuals with larger body size(large body size group)and three individuals with smaller body size(small body size group)were selected by measuring the weight and length of 300 healthy male Alopex lagopus aged 9 months.The pituitary tissues of these individuals were subjected to transcriptome sequencing using the Illumina NovaSeq 6000 platform.Differentially expressed genes(DEGs)were identified based on the sequencing results.GO functional and KEGG pathway enrichment analysis,as well as PPI network analysis,were conducted on the DEGs to screen the genes related to body size variation in Alopex lagopus.Real-time fluorescence quantitative PCR was used to verify the candidate genes.The results showed that a total of 413 DEGs were identified,including 248 expression up-regulated and 165 expression down-regulated DEGs in the large body size group.These DEGs were enriched in 65 GO terms,including single biological processes,cell component organization and biogenesis,cell membrane components,signal transduction activities,which were related to body size variation.The genes involved included IHH,GHSR,GREM1,and SSTR5.They were also enriched in 217 KEGG pathways,including neuroactive ligand-receptor interaction(GHSR,SSTR5 genes),cAMP signaling pathway(GHSR,SSTR5 genes),hedgehog signaling pathway(IHH gene),and TGF-β signaling pathway(GREM1 gene),which were related to body size variation.GHSR,SSTR5,and SST genes were located at the core nodes of the PPI network.Real-time fluorescence quantitative PCR verification showed that the relative expression levels of IHH and GHSR genes were significantly higher than those in the small body size group(P<0.01),while the relative expression levels of GREM1 and SSTR5 genes in the large body size group were significantly lower than those in the small body size group(P<0.01),which was consistent with the results of transcriptome sequencing.The results indicated that genes such as IHH,GHSR,GREM1,and SSTR5 in the pituitary gland of Alopex lagopus might be related to their body size variation.关键词
北极狐/垂体/生长发育/转录组测序/差异表达基因Key words
Alopex lagopus/pituitary/growth and development/transcriptome sequencing/differentially expressed genes分类
农业科技引用本文复制引用
刘庆,李佳鹏,王晨阳,王雨萌,马泽芳,周李生,崔凯..基于垂体转录组测序技术的北极狐体型变异相关基因筛选[J].黑龙江畜牧兽医,2026,(4):155-162,8.基金项目
农业部财政项目"北美赤狐等3个品种性能测定和样品采集"(19220703) （19220703）
青岛农业大学横向课题"改良型蓝狐综合培育技术的开发"(6602419022) （6602419022）
