黑龙江畜牧兽医Issue(4)：92-98,7.DOI:10.13881/j.cnki.hljxmsy.2025.05.0034
黄芩苷对伪狂犬病毒感染的大鼠睾丸及血睾屏障的影响
Effects of baicalin on the testicular and blood-testis barrier of rats infected with Pseudorabies virus
摘要
Abstract
In order to investigate the effects of baicalin on testicular tissue and the blood-testis barrier(BTB)in rats infected with Pseudorabies virus(PRV),30 SPF-grade 7-week-old SD rats were randomly divided into five groups(n=6 per group),which were the Control group,PRV group,low-dose baicalin group(BAI-L,40 mg/kg),medium-dose baicalin group(BAI-M,80 mg/kg),and high-dose baicalin group(BAI-H,160 mg/kg).Rats in the Control and PRV groups were orally administered 2 mL of PBS daily for 14 consecutive days,while the BAI-L,BAI-M,and BAI-H groups received 2 mL of baicalin solution at doses of 40,80,and 160 mg/kg,respectively.On day 15,all groups except the Control group were intranasally inoculated with 60 μL of PRV-XD-F3 suspension,whereas the Control group received an equal volume of normal saline.Subsequently,the Control and PRV groups continued to receive 2 mL of PBS daily for three days,while the BAI groups were administered their respective doses of baicalin solution.On day 17,all rats were euthanized,and blood and testicular samples were collected.Testicular tissue damage was assessed by H.E.staining.Inflammatory cytokines,antioxidant indexes,and testicular activity-related enzymes in testicular tissue were measured using assay kits,and serum testosterone levels were quantified.Viral load in testicular tissue was determined by quantitative real-time PCR.Immunohistochemistry was performed to evaluate the expression of BTB-associated proteins.The results showed that compared with the Control group,the PRV group exhibited a reduction in leydig cells,significantly enlarged intercellular spaces,and cavity formation.All BAI groups showed larger interstitial spaces than the Control group,but smaller spaces than the PRV group,with the BAI-H group demonstrating the most pronounced improvement.Compared with the Control group,the PRV group displayed significantly increased levels of interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),malondialdehyde(MDA),and reactive oxygen species(ROS)concentration(P<0.05),along with significantly decreased levels of interleukin-10(IL-10),superoxide dismutase(SOD)activity,testicular activity-related enzymes acid phosphatase(ACP),alkaline phosphatase(ALP),glucose-6-phosphate dehydrogenase(G-6-PD),lactate dehydrogenase isoenzyme X(LDH-X),and testosterone concentration(P<0.05).Compared with the PRV group,the mass concentrations of IL-1β and IL-6 in BAI-L and BAI-M groups significantly decreased(P<0.05)and that of IL-10 significantly increased(P<0.05).The mass concentrations of IL-1β,IL-6,and TNF-α in BAI-H group significantly decreased(P<0.05)and that of IL-10 significantly increased(P<0.05).All baicalin-treated groups(BAI-L,BAI-M,and BAI-H)demonstrated significantly reduced MDA concentration and ROS concentration(P<0.05),and SOD activity significantly increased(P<0.05).Furthermore,these levels of ACP,G-6-PD,LDH-X,and testosterone in baicalin-treated groups(BAI-L,BAI-M,and BAI-H)significantly increased(P<0.05),along with a significant reduction in PRV copy number(P<0.05).The BAI-M group also exhibited a significant increase in ALP levels(P<0.05).Baicalin increased the expression of ZO-1,Claudin-11,Connexin-43,and N-Cadherin proteins in the blood-testis barrier following PRV infection.The results suggested that baicalin might effectively alleviate PRV-induced viral testicular damage by inhibiting viral replication,reducing inflammatory responses and oxidative stress,and repairing BTB integrity.关键词
伪狂犬病毒/黄芩苷/睾丸损伤/血睾屏障/炎症因子/抗氧化指标Key words
Pseudorabies virus/baicalin/testicular damage/blood-testis barrier/inflammatory factor/antioxidant index分类
农业科技引用本文复制引用
李建芹,廖秀珍,李芯瑞,杨光海,舒相华..黄芩苷对伪狂犬病毒感染的大鼠睾丸及血睾屏障的影响[J].黑龙江畜牧兽医,2026,(4):92-98,7.基金项目
国家重点研发专项省部联动项目"镇雄县昭通乌金猪绿色健康养殖技术集成与示范"(2022YFD1100407) （2022YFD1100407）
云南省农业重大科技专项计划"云南省猪重要疫病防控技术体系构建及应用"(202102AE090007) （202102AE090007）
