河南农业科学2026，Vol.55Issue(3)：61-70,10.DOI:10.15933/j.cnki.1004-3268.2026.03.007
金铃子GATA家族成员鉴定及其在盐胁迫下的功能分析
Identification of GATA Family Members in Momordica charantia and Analysis of Their Functions under Salt Stress
摘要
Abstract
Momordica charantia is a crop with significant economic and medicinal value.However,research on the distribution and stress resistance characteristics of the GATA gene family in Momordica charantia is still limited.This study identified and functionally analyzed the GATA gene family(McaGATA)in Momordica charantia through bioinformatics analysis.Results indicated that a total of 22 McaGATA genes were identified,distributed across 14 chromosomes,and divided into 4 subfamilies based on phylogenetic analysis.The analysis of the physicochemical properties of McaGATA proteins showed that there were differences in the number of amino acids,molecular mass,isoelectric point,etc.,but they were all hydrophilic nuclear proteins.Cis-acting element analysis revealed that the promoter region of the McaGATA genes were rich in light response,hormone response,and stress response elements.qRT-PCR analysis indicated that McaGATA genes presented tissue-specific expression patterns in different tissues,and genes such as McaGATA9,McaGATA10,McaGATA15,McaGATA17 and McaGATA18 were induced by salt stress,showing significantly upregulated expression under low-concentration salt stress conditions.Furthermore,the yeast model was used to verify that McaGATA4,McaGATA15,McaGATA17 and McaGATA18 genes could significantly enhance the salt tolerance of yeast cells under low-concentration salt stress,while McaGATA18 showed the most prominent salt tolerance effect under high salt stress.关键词
金铃子/GATA基因家族/盐胁迫/酵母Key words
Momordica charantia/GATA gene family/Salt stress/Yeast分类
农业科技引用本文复制引用
张晓霞,王金库,冯松浩,王亚旭,娄丽芳..金铃子GATA家族成员鉴定及其在盐胁迫下的功能分析[J].河南农业科学,2026,55(3):61-70,10.基金项目
河南省科技攻关项目(222102310359) （222102310359）
河南省高等学校重点科研项目(25B360007) （25B360007）
