核农学报2026，Vol.40Issue(5)：932-939,8.DOI:10.11869/j.issn.1000-8551.2026.05.0932
甘肃淫羊藿叶斑病的病原鉴定与生物学特性
Identification and Biological Characteristics of The Pathogens of Epimedium brevicomu Leaf Sopts in Gansu Province
摘要
Abstract
Leaf spots disease occurred in Epimedium brevicomu planting area of Weiyuan County,Dingxi City,Gansu Province,seriously affected the yield and quality of E.brevicomu.In order to explore the pathogenic species of E.brevicomu leaf spots,morphological characteristics combined with multi-gene(ITS,TUB2,ACT,GAPDH,CHS-1)molecular biology were conducted.The mycelial growth rate method was used to study biological characteristics.At the same time,controlled indoor inoculations on detached leaves from Leguminosae were performed to identify the potential hosts for pathogen infection.The results of Koch's postulates showed that 10 strains of fungi exhibited same morphological characteristics were etiological agents of E.brevicomu leaf spots.Based on morphological and multi-gene sequences analysis,the representative strain YYH-2 was identified as Colletotrichum incanum.The optimum temperature for strain growth was 25 to 28℃,the optimum pH was 8.0,the optimum carbon source and nitrogen source were soluble starch and beef extract,respectively,and light exposure was beneficial for mycelial growth.Under artificial inoculation conditions,the pathogen can infect the leaves of soybean(Glycine max),broad bean(Vicia faba)and cowpea(Vigna unguiculata).It was the first report of C.incanum as a novel anthracnose pathogen capable of infecting E.brevicornum and cause anthracnose under natural conditions.This study also identified V.faba and V.unguiculata as potential hosts of this pathogen,and highlighted C.incanum's low environmental requirements and robust adaptability.This study provides a theoretical guidance for the diagnosis and subsequent prevention and control of E.brevicomu leaf diseases.关键词
淫羊藿/炭疽病/Colletotrichum incanum/生物学特性/潜在寄主Key words
Epimedium brevicomu/anthracnose/Colletotrichum incanum/biological characteristics/potential hosts引用本文复制引用
蒋晶晶,余海涛,张东佳,师立伟,杜蕙,漆永红,李继平..甘肃淫羊藿叶斑病的病原鉴定与生物学特性[J].核农学报,2026,40(5):932-939,8.基金项目
甘肃省农业科学院农业科技创新专项(2024GAAS21),国家药品监督管理局中药材及饮片质量控制重点实验室项目(2023GSMPA-KL18),甘肃省药品监督管理局药品科研项目(2023GSMPA032),甘肃省林业和草原科技创新项目(LCKJCX202206) （2024GAAS21）
