环球中医药2026，Vol.19Issue(3)：512-520,9.DOI:10.3969/j.issn.1674-1749.2026.03.013
参芪黄颗粒对脓毒症大鼠胃肠功能障碍和肠道菌群的影响
Effects of Shenqihuang Granules on gastrointestinal dysfunction and gut microbiota in sepsis model rats
摘要
Abstract
Objective To investigate the efficacy of Shenqihuang Granules in alleviating gastrointestinal dysfunction in sepsis model rats.Methods The cecal ligation and puncture(CLP)method was used to establish a sepsis rat model.Rats were randomly divided into the sham-operation group,the CLP group,the low dose Shenqihuang group,the medium dose Shenqihuang group,the high dose Shenqihuang group and the dexamethasone group.After modeling,animals were treated for three consecutive days.Rats in the low dose Shenqihuang group,the medium dose Shenqihuang group,the high dose Shenqihuang group were administered Shenqihuang Granules by gavage.The dexamethasone group received dexamethasone by gavage,and the remaining groups were given an equal volume of distilled water by gavage.Enzyme-linked immunosorbent assay was used to detect serum interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),anti-inflammatory factors interleukin-10(IL-10)and arginase-1(Arg-1),and intestinal injury markers diamine oxidase(DAO)and D-lactic acid(D-Lac).Hematoxylin-Eosin(HE)staining was used to observe the histopathological changes in colonic tissue.The gut microbiome is sequenced using metagenomics to analyze the compositional differences in bacterial phyla across groups and identify characteristic microbes.Results Shenqihuang Granules significantly down-regulated serum pro-inflammatory cytokines IL-6 and TNF-α(P<0.01,P<0.05)and elevated anti-inflammatory factors IL-10 and Arg-1(P<0.01,P<0.05).It also improved colon histopathological changes,down-regulated intestinal injury markers DAO and D-Lac(P<0.01),and modulated gut microbiota to restore microbial balance.Conclusion Shenqihuang Granules offer therapeutic benefits for sepsis rats by regulating inflammation,alleviating intestinal injuries,and modulating gut microbiota.关键词
参芪黄颗粒/脓毒症/胃肠功能障碍/肠道菌群/盲肠结扎穿刺法Key words
Shenqihuang Granules/sepsis/gastrointestinal dysfunction/gut microbiota/the cecal ligation and puncture method分类
医药卫生引用本文复制引用
郭星云,梁腾霄,王柏欣,魏曙光,乔阳至子,李珊,陈晴晴,薛静雯,汪宁,颜博涛..参芪黄颗粒对脓毒症大鼠胃肠功能障碍和肠道菌群的影响[J].环球中医药,2026,19(3):512-520,9.基金项目
第五批全国中医临床优秀人才研修项目(国中医药人教函[2022]1号) （国中医药人教函[2022]1号）
陕西省市级中医医院中医药科研能力提升项目(SZY-NLTL-2024-003) （SZY-NLTL-2024-003）
北京中医药大学东直门医院科技创新专项(DZMKJCX-2024-011) （DZMKJCX-2024-011）
北京中医药大学基本科研业务费项目(2024-BUCMXJKY-056,2024-BUCMXJKY-057) （2024-BUCMXJKY-056,2024-BUCMXJKY-057）
