集美大学学报（自然科学版）2026，Vol.31Issue(2)：127-138,12.DOI:10.19715/j.jmuzr.2026.02.01
白棘三列海胆苗种繁育及盐度对受精、胚胎和幼体发育的影响
Seed Production of the Sea Urchin Tripneustes gratilla and Effects of Salinities on Fertilization,Embryo,and Larval Development
摘要
Abstract
To develop aquaculture resources for Tripneustes gratilla(white-spined sea urchin)in China,this study aimed to improve the breeding process for this species.The fertilization,embryo,and larval development processes were observed,and the effects of salinity changes on fertilization,embryo,and larval development were also preliminarily explored.The results are as follows:1)Through artificial insemination,the fertilization rate reached 91.4%.The embryonic development process included the multicellular stage,blastula stage,and gastrula stage,with a hatching rate of 69.2%.Going through multiple stages of breeding,1.5×106 prism larvae develop into four-arm larva,and 1×106 four-larvae develop into to eight-arm larvae,lasting for approximately 50 days,and finally cultivated the juvenile sea urchins of metamorphosis;2)At salinity levels of 30-40,the fertilization rate of T.gratilla exceeded 80%,significantly higher than that in the low salinity groups(20 and 25)(P＜0.05).The best fertilization and hatching performance were observed at 35 salinity.When salinity fell below 25,fertilization rates declined,hindering subsequent development,with no fertilization occurring at 20.The suitable salinity range for the arm larval stage is 35-40;larval growth was better at 35 than at 40,but the survival rate of larvae was sig-nificantly higher at 40 than at 35(P＜0.05).In summary,this study systematically observed the developmental process of T.gratilla and explored the effects of salinity on larval development.The data obtained can provide support for artificial breeding and large-scale aquaculture of sea urchins.关键词
白棘三列海胆/人工繁育/胚胎发育/幼体发育/盐度Key words
Tripneustes gratilla/artificial breeding/embryo development/larval development/salinity分类
农业科技引用本文复制引用
王志远,张丽莉,王国栋..白棘三列海胆苗种繁育及盐度对受精、胚胎和幼体发育的影响[J].集美大学学报（自然科学版）,2026,31(2):127-138,12.基金项目
国家重点研发计划"蓝色粮仓科技创新"重点专项(2018YFD0901605) （2018YFD0901605）
厦门市海洋渔业局(南方海洋中心)(23YYST076QCB30) （南方海洋中心）
福建省海洋与渔业局(FJHY-YYKJ-2023-1-6) （FJHY-YYKJ-2023-1-6）
