集美大学学报（自然科学版）2026，Vol.31Issue(2)：164-175,12.DOI:10.19715/j.jmuzr.2026.02.04
miR-34c-5p介导姜黄素降低HepG2脂质堆积的作用
miR-34c-5p Mediated Curcumin's Effect on Reducing Lipid Accumulation in HepG2 Cells
摘要
Abstract
In order to explore curcumin's regulatory mechanism on lipid metabolism at the transcriptional level,free fatty acid-induced HepG2 cells were used as the model in this study,and cell transfection was em-ployed to adjust the intracellular miRNA levels to address whether curcumin ameliorated hepatic lipid metabolic disorders via miRNA modulation.The results demonstrated that curcumin upregulated the expression of miR-34c-5p.Overexpression of miR-34c-5p led to decreased lipid accumulation in cells,reduced content of triglyc-eride and total cholesterol,as well as downregulated expression of fatty acid and cholesterol synthesis genes,and upregulated expression of fatty acid and triglyceride catabolism genes.Further investigation revealed that some genes involved in fatty acid synthesis and elongation and glycerolipid metabolism pathways were regulated by miR-34c-5p.In conclusion,curcumin alleviated lipid metabolic disorders by increasing miR-34c-5p,thereby reducing lipid accumulation in HepG2 cells.关键词
微小RNA/姜黄素/脂质代谢/肝癌细胞HepG2/转录调控Key words
miRNA/curcumin/lipid metabolism/HepG2 cells/transcriptional regulation分类
轻工纺织引用本文复制引用
武筱雯,王浩宇,林彦祺,付凯丽,邵鹏,林静瑜,李桂玲..miR-34c-5p介导姜黄素降低HepG2脂质堆积的作用[J].集美大学学报（自然科学版）,2026,31(2):164-175,12.基金项目
国家自然科学基金项目(31771972) （31771972）
福建省自然科学基金项目(2017J01447) （2017J01447）
