南京师大学报（自然科学版）2026，Vol.49Issue(2)：49-58,10.DOI:10.3969/j.issn.1001-4616.2026.02.005
共培养体系下融合魏斯氏菌XG-3胞外多糖的产量优化及特性研究
Optimization of Exopolysaccharide Production by Weissella confusa XG-3 in a Co-culture System and Characterization of its Properties
摘要
Abstract
In this study,a co-culture system integrating Weissella confusa XG-3 and Candida shehatae was established.The One-Factor-At-A-Time method was used to optimize exopolysaccharide production by XG3,and the properties of the produced EPS were investigated.The results demonstrated that when C.shehatae was inoculated 4 h after W.confusa XG-3 at an inoculation ratio of 1∶1,the EPS yield reached its maximum(56.82±0.12)g/L,which was 1.63 times higher than that obtained from monoculture.The optimal medium formulation for EPS production was composed of 7%sucrose,0.3%yeast extract,1.2%peptone,0.03%magnesium sulfate,0.4%ammonium citrate,0.3%dipotassium phosphate,0.05%Tween-80,5%sodium acetate,and 10%beef extract.The optimal culture conditions were an initial pH of 6.0,an incubation temperature of 30℃,and a shaking speed of 120 rpm.Based on optimization,the EPS yield of the W.confusa XG-3 strain reached(89.32±0.55)g/L,representing a 2.6-fold increase compared to before optimization.The EPS produced under co-culture exhibited excellent emulsifying activity(the emulsification indices for soybean oil and gasoline were(92.65±0.07)%and(81.08±0.09)%,respectively),a high intrinsic viscosity,and strong heavy-metal adsorption capacity.关键词
共培养/融合魏斯氏菌/胞外多糖/优化/性质Key words
co-culture/Weissella confusa/exopolysaccharide/optimization/properties分类
生物科学引用本文复制引用
曹文杰,赵守祺,高古博,杨舒涵,杜仁鹏,赵丹..共培养体系下融合魏斯氏菌XG-3胞外多糖的产量优化及特性研究[J].南京师大学报（自然科学版）,2026,49(2):49-58,10.基金项目
国家自然科学基金项目(32072189)、黑龙江省高校基本科研业务费科研项目(2024-kYYWF-0127). （32072189）
