农药学学报2026，Vol.28Issue(2)：269-276,8.DOI:10.16801/j.issn.1008-7303.2026.0022
莲缢管蚜气味结合蛋白9的鉴定及与8种杀虫剂的结合特性
Identification of odorant binding protein 9 in Rhopalosiphum nymphaeae and its binding characteristics with eight insecticides
摘要
Abstract
Rhopalosiphum nymphaeae is a major insect pest of aquatic vegetables and is capable of causing yield losses exceeding 50%under severe infestation.In recent years,there has been an increasing number of reports on odorant binding proteins(OBPs)mediated pesticide resistance in insects.To elucidate the binding characteristics of R.nymphaeae OBPs towards pesticides,this study cloned RnymOBP9 gene.An integrated strategy involving phylogenetic analysis,quantitative real-time PCR(RT-qPCR),and fluorescent competitive binding assays was employed to investigate its tissue-specific expression profiles and binding affinity with eight pesticides and the aphid alarm pheromone,(E)-β-farnesene(EBF).Results showed that RnymOBP9 gene(GenBank accession number:PX123855)contains an open reading frame(ORF)of 384 bp,encoding a protein of 127 amino acids.Phylogenetic analysis revealed that RnymOBP9 is closely related to other aphid OBP9 orthologs.Tissue expression profiling indicated that RnymOBP9 is expressed in multiple tissues,with notably high and specific expression in the antennae.When the recombinant RnymOBP9 protein was bound to the N-phenyl-1-naphthylamine(1-NPN)fluorescent probe,the addition of imidacloprid,dinotefuran,and EBF reduced the relative fluorescence intensity(RFI)to below 50%.Notably,EBF exhibited the strongest binding affinities to OBP9,with dissociation constants(Kd)of(6.389±0.596)μmol/L,followed by dinotefuran,with a Kd of(10.510±0.754)μmol/L.These findings demonstrate that RnymOBP9 binds both EBF and synthetic insecticides,suggesting its potential involvement in key biological processes such as chemical signal recognition and the mediation of insecticide resistance in R.nymphaeae.关键词
莲缢管蚜/气味结合蛋白/组织表达谱/吡虫啉/呋虫胺/荧光竞争结合Key words
Rhopalosiphum nymphaeae/odorant binding protein/tissue expression profile/imidacloprid/dinotefuran/fluorescent competitive binding assays分类
农业科技引用本文复制引用
高云,马丽君,周福才,刘航玮,杨新玲,罗晨..莲缢管蚜气味结合蛋白9的鉴定及与8种杀虫剂的结合特性[J].农药学学报,2026,28(2):269-276,8.基金项目
国家特色蔬菜产业技术体系(No.CARS-24-C-03). Supported by National Special Vegetable Industry Technology System of China(No.CARS-24-C-03). （No.CARS-24-C-03）
