农药学学报2026，Vol.28Issue(2)：312-320,9.DOI:10.16801/j.issn.1008-7303.2026.0013
冻土毛霉可湿性粉剂的研制及对茴香薄翅野螟的生物活性
Development of Mucor hiemalis wettable powder and its biological activity against Evergestis extimalis Scopoli
摘要
Abstract
To further enhance the biological activity of Mucor hiemalis against Evergestis extimalis Scopoli,single-factor experiment and orthogonal array design were used to identify the carriers and additives with minimal impact on the viability of M.hiemalis QH01.An optimized formulation of M.hiemalis wettable powder(WP)was established.The results showed that the optimum formulation of the WP consisted of 1×108 spores/mL,30%white carbon black(carrier),4%sodium carboxymethyl cellulose(CMC-Na,dispersant),2%polyethylene glycol 6000(PEG6000,wetting agent),and 1%ascorbic acid(VC,ultraviolet protectant).The WP exhibited a suspension of 85.03%,a wetting time of 56 s,a pH value of 7.05,a fineness of 97.52%,and a water content of 2.33%.Stability test revealed no significant change in the viable bacteria count of the WP at the temperature ranging of 20 to 40℃,or the pH value ranging from 6 to 8 and the spore concentration can reach 1×108 spores/mL(P>0.05).After 120 minutes of ultraviolet radiation,it still retains a certain level of activity(spore concentration of 0.28×108 spores/mL).After 45 days of indoor storage,the spore concentration decreased slightly but remained at 0.77×108 spores/mL,indicating good stability.The results of indoor bioassay showed that after 5 days of treatment with 1×108 spores/mL of the WP of Mucor hiemalis,the median lethal time(LT50)value for the 3rd instar larvae of Evergestis extimalis Scopoli was the lowest,at 2.1 days,highlighting its promising potential for practical application.关键词
冻土毛霉/可湿性粉剂/制剂制备/茴香薄翅野螟/生物防治Key words
Mucor hiemalis/wettable powder(WP)/preparation of preparations/Evergestis extimalis Scopoli/biological control分类
农业科技引用本文复制引用
王沙沙,安泽豪,李美琪,来有鹏..冻土毛霉可湿性粉剂的研制及对茴香薄翅野螟的生物活性[J].农药学学报,2026,28(2):312-320,9.基金项目
青海省科技厅应用基础研究项目(No.2023ZJ739) （No.2023ZJ739）
青海省海南藏族自治州科技计划项目(No.2025-KZ03-E). Supported by Qinghai Provincial Science and Technology Department Applied Basic Research Project(No.2023ZJ739) （No.2025-KZ03-E）
Qinghai Hainan Tibetan Autonomous Prefecture Science and Technology Plan Project(No.2025-KZ03-E). （No.2025-KZ03-E）
