日用化学品科学2026，Vol.49Issue(4)：19-26,40,9.DOI:10.3969/j.issn.1006-7264.2026.04.004
原花青素B2通过修复线粒体功能障碍拮抗多柔比星诱导的细胞衰老
Proanthocyanidin B2 mitigates doxorubicin-induced cellular senescence by restoring mitochondrial function
摘要
Abstract
The protective effects of proanthocyanidin B2(PB2)against doxorubicin(DOX)-induced mitochondrial dysfunction and cellular senescence in human skin fibroblasts(HSFs)was investigated.A DOX-induced HSF senescence model was established,and cells were treated with varying concentrations of PB2.Reactive oxygen species(ROS)levels and mitochondrial membrane potential(ΔΨm)were assessed using fluorescence microscopy.ATP content was measured using commercial assay kits,DNA damage was evaluated via γ-H2AX immunofluorescence,and senescence-associated β-galactosidase(SA-β-gal)staining was used to characterize senescent phenotypes.PB2 treatment effectively mitigated DOX-induced cellular damage.Among the concentrations tested,2 μmol/L PB2 showed the most pronounced effect,reducing intracellular ROS accumulation by over 50%,restoring mitochondrial function(ΔΨm increased 8.75-fold),and enhancing ATP production by 33.08%.Moreover,γ-H2AX expression decreased by 25.53%,and SA-β-gal activity was inhibited by 38.20%.These findings indicate that PB2 delays DOX-induced cellular senescence by scavenging ROS,restoring mitochondrial function(ΔΨm homeostasis and ATP synthesis),and alleviating DNA damage,providing a theoretical basis for the development of PB2-based anti-aging cosmetic products.关键词
原花青素B2/抗衰老/线粒体功能/活性氧/DNA损伤/人皮肤成纤维细胞Key words
proanthocyanidin B2/anti-aging/mitochondrial function/reactive oxygen species/DNA damage/human skin fibroblasts分类
化学化工引用本文复制引用
李广涛,胡昕,高宏旗,赖志芳,袁婵龄,梅文杰..原花青素B2通过修复线粒体功能障碍拮抗多柔比星诱导的细胞衰老[J].日用化学品科学,2026,49(4):19-26,40,9.基金项目
广东省自然科学基金项目(2024A1515013282) （2024A1515013282）
