世界中西医结合杂志2026，Vol.21Issue(2)：258-264,7.DOI:10.13935/j.cnki.sjzx.260207
增液通腑逐瘀方调控cGAS-STING信号通路改善脓毒症大鼠肠道通透性作用机制
Mechanism of Zengye Tongfu Zhuyu Prescription in Regulating cGAS-STING Signaling Pathway to Improve Intestinal Permeability in Septic Rats
摘要
Abstract
Objective To study the improvement of intestinal permeability in septic rats by Zengye Tongfu Zhuyu Prescription.Methods The model of septic rats was established by cecal ligation and puncture(CLP).A total of 36 healthy SPF grade male SD rats were randomly divided into a sham group,a model group,and low-and high-dose Zengye Tongfu Zhuyu Prescription groups using a random number table method,with 9 rats in each group.After 3 days of continuous administration,they were sacrificed,and ileal tissues were collected and prepared for pathological sections.The degree of intestinal injury was determined using the Chiu's score.Western blot was employed to detect the expression of intestinal cyclic GMP-AMP synthase(cGAS),stimulator of interferon genes(STING),myosin light chain kinase(MLCK),occludin,and zonula occludens-1(ZO-1).The messenger ribonucleic acid(mRNA)expression of intestinal tumor necrosis factor-α(TNF-α)and interferon-α(IFN-α)was measured by real-time quantitative reverse transcription polymerase chain reaction(RT-qPCR).Intestinal permeability was measured by the lipopolysaccharide(LPS)method.Results Compared with the sham group,the model group showed significantly increased Chiu's score,relative expression of cGAS,STING,and MLCK,and mRNA relative expression of TNF-α and IFN-α(P<0.01)and significantly decreased relative expression of occludin and ZO-1(P<0.01).Compared to the model group,the low-dose Zengye Tongfu Zhuyu Prescription group exhibited significantly decreased Chiu's score,relative expression of MLCK,mRNA relative expression of IFN-α,and LPS content(P<0.05 or P<0.01)and significantly increased relative expression level of ZO-1(P<0.01).As for the high-dose Zengye Tongfu Zhuyu Prescription group,the Chiu's score,relative expression of cGAS,STING,and MLCK,mRNA relative expression of TNF-α and IFN-α,and LPS content were significantly decreased(P<0.01),while the relative expression of occludin and ZO-1 was significantly increased(P<0.01).Conclusion The Zengye Tongfu Zhuyu Prescription can inhibit the activation of the cGAS-STING pathway,suppress type I interferon(IFN-I)response and the release of inflammatory mediators,and reduce the change of intestinal tight junctions(TJs),thereby improving intestinal permeability and maintaining the integrity of intestinal mucosal barrier.关键词
脓毒症/增液通腑逐瘀方/肠道通透性/cGAS-STING信号通路/肠上皮细胞Key words
sepsis/Zengye Tongfu Zhuyu Prescription/intestinal permeability/cGAS-STING signaling pathway/intestinal epithelial cells分类
医药卫生引用本文复制引用
高薇薇,高万朋,关鹏..增液通腑逐瘀方调控cGAS-STING信号通路改善脓毒症大鼠肠道通透性作用机制[J].世界中西医结合杂志,2026,21(2):258-264,7.基金项目
天津市教委科研计划项目(自然科学)(2022KJ185) （自然科学）
天津市卫生健康科技项目(TJWJ2024MS014) （TJWJ2024MS014）
