沈阳医学院学报2026，Vol.28Issue(2)：126-130,156,6.DOI:10.16753/j.cnki.1008-2344.2026.02.003
磷酸二酯酶3特异性抑制剂米利酮体外对人成熟精子运动能力的影响
Effects of phosphodiesterase 3 specific inhibitor milrinone on the motility of human mature sperm in vitro
摘要
Abstract
Objective:To investigate the effects of phosphodiesterase 3(PDE3)specific inhibitor milrinone on the motility of sperm from healthy individuals and asthenozoospermic patients in vitro.Methods:Sperm from healthy individuals and asthenozoospermic patients were treated in vitro with 10 μmol/L milrinone for 1 h.The two types of sperm without milrinone treatment served as the control groups.Sperm motility parameters were assessed using a computer-assisted sperm analysis(CASA)system.Results:Compared with the control,milrinone had no effect on total motility,progressive motility,non-progressive motility and motility parameters in healthy sperm(P＞0.05).Compared with the control in asthenozoospermic samples,milrinone significantly increased the total motility,progressive motility,non-progressive motility,and also enhanced the mean curvilinear velocity,mean straight-line velocity,average path velocity,and mean beat-cross frequency of sperm(P＜0.05).There were no statistically significant differences between the two groups in terms of sperm lateral head displacement and movement patterns(mean amplitude of lateral head displacement,mean angular displacement,linearity,wobble and straightness)(P＞0.05).Conclusion:Milrinone can effectively activate sperm motility in asthenozoospermic patients in vitro.关键词
米利酮/环磷酸腺苷/磷酸二酯酶/精子活力Key words
milrinone/cyclic adenosine monophosphate/phosphodiesterase/sperm motility分类
医药卫生引用本文复制引用
徐陆陈,邹乾兴,赵早亚..磷酸二酯酶3特异性抑制剂米利酮体外对人成熟精子运动能力的影响[J].沈阳医学院学报,2026,28(2):126-130,156,6.基金项目
国家自然科学基金项目(No.82360293) （No.82360293）
广西科技计划项目(No.桂科AD23026059) （No.桂科AD23026059）
广西高校中青年教师科研基础能力提升项目(No.2023KY0101) （No.2023KY0101）
柳州市科技计划项目(No.2024YB0101A006) （No.2024YB0101A006）
广西青年科技人才托举工程项目(No.GXYESS2025126) （No.GXYESS2025126）
