西北农林科技大学学报（自然科学版）2026，Vol.54Issue(4)：33-42,10.DOI:10.13207/j.jnwafu.2026.04.005
7个白杨无性系耐低氮性综合评价
Comprehensive evaluation of low nitrogen tolerance in 7 poplar clones
摘要
Abstract
[Objective]This research aimed to explore the growth and physiological response of 7 poplar clones under low nitrogen stress,and to select excellent poplar clones with strong low nitrogen tolerance from them.[Method]A pot experiment was conducted on 7 poplar clones,including 84K,Maobaiyang 30,Qinbai-yang 1,Qinbaiyang 3,Qinbaiyang 5,and Xibeiyang 1 and Xibeiyang 3.Seedlings were subjected to two nitrogen treatments:1 mmol/L NH₄NO₃(control,CK)and 0.01 mmol/L NH₄NO₃(low nitrogen,LN).After 60 days of treatment,growth and physiological parameters of poplar clones seedling were measured.Comprehensive evaluation of low nitrogen tolerance was conducted using correlation analysis,principal component analysis,and membership function analysis.[Result]Compared with CK,the growth indicators(seedling height increment,ground diameter increment,plant biomass),chlorophyll content,net photosynthetic rate,nitrogen accumulation(NH4+-N,NO3--N,NO2--N),and the activities of various enzymes(nitrate reductase(NR),nitrite reductase(NiR),glutamate synthase(GOGAT),and glutamine synthase(GS))of 7 poplar clones under low nitrogen treatment were significantly reduced.Under low nitrogen stress,the decrease in NH4+-N content in the leaves and roots of 7 poplar clones seedlings was significantly higher than that of NO3--N,NO2--N,indicating that the 7 clones were more inclined towards ammonium loving tree species.The correlation analysis results showed that there was a significant correlation between the growth and physiological indicators of poplar clonal seedlings.Principal component analysis integrated all measurement indicators into three component factors and divided them into nitrogen accumulation factors and nitrogen metabolism factors.The results of the membership function analysis showed that the order of low nitrogen tolerance of the 7 tested poplar clones from strong to weak was 84K>Xibeiyang 3>Xibeiyang 1>Qinbaiyang 1>Qinbaiyang 5>Qinbaiyang 3>Maobaiyang 30.[Conclu-sion]Among the 7 clones of poplar seedlings,84K,Xibeiyang 3,and Xibeiyang 1 have strong low nitrogen toler-ance and can be used as the main promotion and artificial afforestation tree species in soil poor areas.关键词
杨树无性系/低氮胁迫/耐低氮性综合评价Key words
poplar clones/low nitrogen stress/comprehensive evaluation of low nitrogen resistance分类
农业科技引用本文复制引用
火占华,黄景宝,邵雅坤,张静,张春霞..7个白杨无性系耐低氮性综合评价[J].西北农林科技大学学报（自然科学版）,2026,54(4):33-42,10.基金项目
"十四五"国家重点研发计划揭榜挂帅项目"渭河平原白杨大径级工业资源材精准高效培育"(2021YFD2201203) （2021YFD2201203）
