西北农林科技大学学报（自然科学版）2026，Vol.54Issue(4)：123-132,10.DOI:10.13207/j.jnwafu.2026.04.014
不同育性枸杞小孢子减数分裂过程及其结构特征差异分析
Analysis of the differences in meiosis process and structural characteristics Lycium barbarum L.microspore with different fertility levels
摘要
Abstract
[Objective]The study aims to systematically compare the correlation between anther morphol-ogy and microspore development in the fertile line'Ningqi 1'and the male sterile line'Ningqi 5',identify the critical stage of microspore abortion,and reveal the differences in ultrastructure between the two lines,thereby providing a theoretical basis for clarifying the molecular regulatory mechanism of male sterility in L.barbarum L..[Method]Flower buds at different developmental stages of the fertile line'Ningqi 1'and the sterile line'Ningqi 5'were used as experimental materials.Phenotypic observations and comparative analyses of micro-spore development were performed.The meiotic process of pollen mother cells and the structural characteristics of microspore abortion were analyzed using the squash methods and transmission electron microscopy.[Result]During the meiosis in pollen mother cells of both lines,chromosome behavior was normal,yielding normal tet-rads.Tetrads in'Ningqi 1'developed into fertile pollen grains filled with starch granules and lipid droplets.In contrast,microspores in'Ningqi 5'underwent progressive shrinkage,deformation,and disintegration within the callose.Ultrastructural analysis revealed that microspores in'Ningqi 5'could form a primary pollen exine within the callose,but the cytoplasm adjacent to the cell wall gradually vacuolated,the nuclei condensed,and eventually the microspores completely disintegrated,leading to pollen abortion.Additionally,the cell walls of the tapetum cells in'Ningqi 5'exhibited abnormal thickening,which indicated abnormal programmed cell death.[Conclu-sion]Anther abortion in sterile lines occurs at the tetrad stage,and the failure of callose degradation around mi-crospores is the direct cause of abortion.It is speculated that abnormal development of tapetum cells results in callase deficiency or insufficient enzyme activity,thereby preventing the release of tetrad microspores and ulti-mately causing male sterility.关键词
宁夏枸杞/雄性不育/小孢子发育/减数分裂Key words
Lycium barbarum L./male sterility/microspore development/meiosis分类
农业科技引用本文复制引用
杨小惠,周婷,魏雅茹,梁文裕,杨淑娟..不同育性枸杞小孢子减数分裂过程及其结构特征差异分析[J].西北农林科技大学学报（自然科学版）,2026,54(4):123-132,10.基金项目
宁夏自然科学基金项目"DYT1对宁夏枸杞花药绒毡层及花粉发育的调控机制研究"(2024AAC03106) （2024AAC03106）
国家自然科学基金项目"雄性不育枸杞绒毡层细胞对育性影响机制的研究"(32060077) （32060077）
