中国动物检疫2026，Vol.43Issue(3)：108-112,5.DOI:10.3969/j.issn.1005-944X.2026.03.016
3种方法对犬猫狂犬病疫苗免疫抗体检测的比较
Comparison of Three Methods for Immune Antibodies Against Rabies Vaccines in Dogs and Cats
杨国强 1吕宗吉 2潘杰 3钟亮宁 3陈燕君 4张险朋 3罗霖霜 1杜艺彤 1邝锦霞 4何铭诗 4何德胜4
作者信息
- 1. 佛山大学,广东佛山 528231
- 2. 佛山大学,广东佛山 528231||广东金维度生命科学技术有限公司,广东佛山 528200
- 3. 东莞市动物疫病预防控制中心,广东东莞 523086
- 4. 广东金维度生命科学技术有限公司,广东佛山 528200
- 折叠
摘要
Abstract
In order to evaluate the performance of commercial kits for immune antibodies against rabies vaccines,and to assess the immune effect of the vaccines,taking fluorescent antibody virus neutralization test(FAVN)as a gold standard,enzyme-linked immunosorbent assay(ELISA)kit A with batch No.of 806F and colloidal gold test strip C with good performance were selected from ELISA kit A with batch Nos.of 307E and 806F,B and strips C and D,through detecting rabies immune antibodies in 20 canine reference serum samples,to detect 219 samples randomly collected from immunized dogs and cats in Dongguan City in combination with FAVN.The results showed that significant differences were observed through the three methods(P＜0.01),the positive detection rate was the highest by FAVN,and the lowest by strip C that was with higher specificity;the differences were more obviously in detection of weakly positive samples,but the concordance rate was higher for negative or strongly positive samples;the positive rates of serum antibodies detected by the three methods were significantly higher in dogs than in cats(P＜0.01),and serum neutralizing antibody titers and antibody positive rates were greatly increased by booster immunization(number of immunization≥2).A reference was provided for selection of methods for detecting immune antibodies against rabies vaccines in dogs and cats,as well as for application of immunization with rabies vaccines.关键词
狂犬病/免疫抗体/检测方法比较/荧光抗体病毒中和试验/酶联免疫吸附试验/胶体金试纸条Key words
rabies/immune antibody/comparison of detection methods/FAVN/ELISA/colloidal gold test strip分类
农业科技引用本文复制引用
杨国强,吕宗吉,潘杰,钟亮宁,陈燕君,张险朋,罗霖霜,杜艺彤,邝锦霞,何铭诗,何德胜..3种方法对犬猫狂犬病疫苗免疫抗体检测的比较[J].中国动物检疫,2026,43(3):108-112,5.
