中国动物检疫2026，Vol.43Issue(3)：113-119,7.DOI:10.3969/j.issn.1005-944X.2026.03.017
新型鹅细小病毒VP3病毒样颗粒的制备及免疫原性鉴定
Preparation and Immunogenicity Identification of VP3 Virus-like Particles of Novel Goose Parvovirus
栾志舫 1康正武 1刘宏 1葛学凤 1王梦惠 1宋庆庆 1涂亚斌1
作者信息
- 1. 山东信得科技股份有限公司,山东青岛 266100
- 折叠
摘要
Abstract
Short beak and dwarfism syndrome(SBDS)in ducks could be caused by novel goose parvovirus(NGPV)that is a non-enveloped and single-stranded DNA virus.In order to prepare NGPV VP3 virus-like particles(VLPs)and evaluate their immunogenicity,VP3 gene of NGPV SD strain genome was amplified by PCR.The gene sequence was subsequently optimized(through codon preference optimization with addition of a translation-enhancing sequence at 5'end of the target gene),and a recombinant strain expressing the VP3 protein was constructed using a molecular chaperone co-expression strategy.The expressed VP3 protein was purified and emulsified for preparation of subunit vaccines.The prepared vaccine samples were inoculated into ducklings for assessment of their immune efficacy.The results showed that uniform VLPs approximately 20 nm in diameter were assembled from the purified VP3 protein,as confirmed by transmission electron microscopy.Immunogenicity analysis revealed that these VLPs exhibited high affinity with NGPV-positive serum(antigen titer 1∶512),and the prepared subunit vaccines induced high levels of neutralizing antibodies in target animals[titer:(6.20±0.65)log2].In conclusion,an efficient process for soluble expression of NGPV VP3 protein and preparation of VLPs was established,laying a foundation for further development and application of NGPV subunit vaccines.关键词
新型鹅细小病毒/VP3蛋白/原核表达/病毒样颗粒Key words
NGPV/VP3 protein/prokaryotic expression/virus-like particle分类
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栾志舫,康正武,刘宏,葛学凤,王梦惠,宋庆庆,涂亚斌..新型鹅细小病毒VP3病毒样颗粒的制备及免疫原性鉴定[J].中国动物检疫,2026,43(3):113-119,7.
