中国动物检疫2026，Vol.43Issue(3)：120-126,7.DOI:10.3969/j.issn.1005-944X.2026.03.018
新疆南美白对虾副溶血性弧菌的分离鉴定
Isolation and Identification of Vibrio parahaemolyticus from Litopenaeus vannamei in Xinjiang
摘要
Abstract
In order to identify infection status of Vibrio parahaemolyticus(V.parahaemolyticus)in Litopenaeus vannamei(L.vannamei)in major farming areas of Xinjiang,187 shrimp samples(including post-larvae,juveniles and adults)were collected from 47 ponds where disease outbreaks were suspected on 12 farms in major production areas of Bayingolin,Changji and Tacheng from June to September 2025,to isolate and purify vibrio species using TCBS agar medium,and to identify bacterial strains through 16S rDNA sequence analysis;concurrently,DNAwas extracted from hepatopancreas,hepatopancreas enrichment broth and the isolated strain for PCR assay to compare and analyze the test results.The results showed that the overall detection rate of V.parahaemolyticus was 21.4％(40/187),with a total of 73 strains isolated;over 99％sequence homology was observed between the isolated strain and the standard strain,attesting that the identification was accurate;based on the results of different samples,the detection rate by PCR was the highest for isolated strain(21.4％),followed by hepatopancreas enrichment broth(19.8％),and then hepatopancreas(9.6％).In conclusion,V.parahaemolyticus infection was available in L.vannamei in Xinjiang;and DNA extracted from hepatopancreas enrichment broth was recommended for PCR assay for samples with low bacterial loads and in rapid screening scenarios.关键词
南美白对虾/副溶血性弧菌/细菌分离鉴定/16S rDNA/新疆Key words
L.vannamei/V.parahaemolyticus/bacterial isolation and identification/16S rDNA/Xinjiang分类
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阿依图玛尔·阿卜杜赛麦提,马玉兰,孙赫,卓越,李志恒,岳城,郝翠兰..新疆南美白对虾副溶血性弧菌的分离鉴定[J].中国动物检疫,2026,43(3):120-126,7.基金项目
新疆维吾尔自治区重点研发任务专项(2025B02020-2,2023B02012) （2025B02020-2,2023B02012）
国家自然科学基金项目(32403085) （32403085）
