中国癌症杂志2026，Vol.36Issue(3)：299-311,13.DOI:10.19401/j.cnki.1007-3639.2026.03.010
肿瘤类器官构建通用规范专家共识(2026年版)
General guidelines for the construction of tumor organoids expert consensus(2026 edition)
摘要
Abstract
Organoid technology,which enables three-dimensional in vitro culture to closely recapitulate key structural and functional features of human tissues and organs,has significant value in disease modeling,precision medicine,and drug discovery and development.With continued advances in organoid research,tumor organoids have been established for a wide range of solid tumors,and the technology was highlighted by The New England Journal of Medicine in 2019 as an excellent preclinical disease model.However,in China,unified technical guidelines and a consensus framework are still lacking for critical aspects of tumor organoid practice,including specimen collection,culture systems,quality control,and compliance governance.This has limited the reproducibility of research findings and their clinical translation.To address these gaps,this consensus was developed by the Organoid Professional Committee of the Shanghai Anticancer Association and Clinical Precision Medicine Professional Committee of Chinese Medical Doctor Association,among others,based on extensive searches of domestic and international literature,evidence grading,and expert review.For the first time,it systematically proposes a full-process general framework for tumor organoid construction,covering specimen collection and processing,organoid culture and passaging,cryopreservation and recovery,and quality control throughout the entire workflow.This consensus is intended to provide a scientific and practical technical guidance framework for researchers and clinicians engaged in tumor organoid studies,improve model fidelity and reliability,facilitate data sharing and cross-study comparability,and accelerate the standardized implementation of organoid technology in precision oncology.The consensus will be updated periodically in accordance with emerging evidence and accumulated practical experience from organoid-based research.关键词
类器官/肿瘤/通用规范/全流程/共识Key words
Organoids/Tumor/General guidelines/End-to-end workflow/Consensus分类
医药卫生引用本文复制引用
上海市抗癌协会类器官专业委员会,中国医师协会临床精准医疗专业委员会,中国医药生物技术协会组织生物样本库分会,中国研究型医院学会临床数据与样本资源库专业委员会,中国抗癌协会类器官与器官芯片专业委员会,中国细胞生物学学会类器官研究与应用工作委员会,中国医药生物技术协会类器官技术临床应用分会,王琦,许蜜蝶..肿瘤类器官构建通用规范专家共识(2026年版)[J].中国癌症杂志,2026,36(3):299-311,13.基金项目
国家自然科学基金(82473011,82273370,U21A20376). National Natural Science Foundation of China(82473011,82273370,U21A20376). （82473011,82273370,U21A20376）
