医药导报2026，Vol.45Issue(4)：609-617,9.DOI:10.3870/j.issn.1004-0781.2026.04.008
基于HPLC-MS/MS同时检测天麻中天麻素和巴利森苷B及建立4种脑内神经递质微透析体内外方法
Simultaneous Detection of Gastrodin,Parishin B in Gastrodia Elata by HPLC-MS/MS and Establishment of an in vitro and in vivo Microdialysis Method for Four Cerebral Neurotransmitters
摘要
Abstract
Objective To establish an in vivo and in vitro microdialysis method for the simultaneous determination of gastrodin(Gas)and parishin B(PB)from Gastrodia elata,as well as four brain neurotransmitters,using high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Methods Both forward(loss)and reverse(gain)dialysis approaches were applied in combination with HPLC-MS/MS to investigate the probe recovery rates of Gas,PB and the neurotransmitters glutamate(Glu),γ-aminobutyric acid(GABA),valine(Val),and 5-hydroxytryptamine(5-HT)in vitro and in vivo.Factors influencing probe recovery,including perfusion flow rate,drug concentration,number of probe uses,and stability,were systematically evaluated to optimize the analytical method.Results The effects of different perfusion flow rates(1.5,2.0 and 2.5 μL•min-1)indicated an inverse relationship between flow rate and probe recovery.Considering sample volume requirements,2.0 μL•min-1 was selected for subsequent experiments.Probe recovery remained consistent across different drug concentrations in both in vitro and in vivo settings,suggesting that recovery is independent of concentration.Furthermore,probe stability was maintained within 8 hours.Each probe could be reused up to three times after prior recovery calibration.Conclusions The established microdialysis method is suitable for conducting pharmacokinetic-pharmacodynamic(PK-PD)studies of gastrodin and parishin B from ultrafine powder of Gastrodia elata in the brain.关键词
天麻素/巴利森苷B/微透析/回收率/神经递质Key words
Gastrodin/Parishin B/Microdialysis/Recovery rates/Neurotransmitters分类
医药卫生引用本文复制引用
肖迪,巩仔鹏,黄远杏,陈艳,金阳,郑林,陈思颖,王爱民,孙宝飞,马致洁..基于HPLC-MS/MS同时检测天麻中天麻素和巴利森苷B及建立4种脑内神经递质微透析体内外方法[J].医药导报,2026,45(4):609-617,9.基金项目
国家重点研发计划项目(2023YFC2308202) （2023YFC2308202）
国家自然科学基金资助项目(81560646) （81560646）
国家大学生创新创业训练计划项目(202210660130). （202210660130）
