医药导报2026，Vol.45Issue(4)：625-632,8.DOI:10.3870/j.issn.1004-0781.2026.04.010
荷叶碱对脂多糖诱导的人脐静脉内皮细胞炎症的影响
Effect of Nuciferine on Lipopolysaccharide-induced Inflammation in Human Umbilical Vein Endothelial Cells
摘要
Abstract
Objective To explore the protective effects of nuciferine against LPS-triggered endothelial inflammation and elucidate the underlying molecular mechanisms in HUVECs.Methods HUVECs were cultured in vitro and pre-treated with escalating concentrations of nuciferine(0.6,1.25,2.5,5,10,20 μmol•L-1)for 24 hours,followed by 12-hour stimulation with LPS(1 μg • mL-1).Cell viability was assessed via CCK-8 assay to determine the optimal nuciferine concentration(10 μmol•L-1),which was then used for subsequent experiments.HUVECs were randomized into four groups:blank control group;nuciferine group(10 μmol•L-1);LPS group(1 μg•mL-1);LPS(1 μg•mL-1)+nuciferine(10 μmol•L-1).Quantitative real-time PCR(qRT-PCR)and enzyme-linked immunosorbent assay(ELISA)were performed to quantify mRNA and protein levels of inflammatory cytokines,including interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α).Western blotting was employed to analyze protein expression of cyclooxygenase-2(COX2),inducible nitric oxide synthase(iNOS),B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax),cleaved caspase-3(c-caspase-3),Toll-like receptor 4(TLR4),IκB kinase β(IKKβ),nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor α(IκBα),and nuclear factor κB p65 subunit(p65).Immunofluorescence staining was used to visualize p65 nuclear translocation.Results Compared with the control group,LPS stimulation significantly reduced cell viability(P<0.01),upregulated mRNA and protein levels of IL-1β,IL-6,and TNF-α,increased protein expression of COX2 and iNOS(P<0.01),and elevated the Bax/Bcl-2 ratio and c-caspase-3 levels(P<0.01).In contrast,co-treatment with nuciferine and LPS significantly attenuated LPS-induced cytotoxicity(P<0.05),suppressed pro-inflammatory cytokine production,mitigated apoptosis,and downregulated the Bax/Bcl-2 ratio and cleaved c-caspase-3 levels(P<0.05).Conclusion Nuciferine exerts protective effects against LPS-induced endothelial inflammation and apoptosis via suppression of the TLR4/NF-κB cascade,offering mechanistic insights and potential therapeutic strategies for sepsis.关键词
荷叶碱/人脐静脉内皮细胞/炎症/凋亡/Toll样受体4/核因子κBKey words
Nuciferine/Human umbilical vein endothelial cells/Inflammation/Apoptosis/Toll-like receptor 4/nuclear factor κB分类
医药卫生引用本文复制引用
张梦,徐飞雪,陈雅婕,沈镯钰,唐其柱..荷叶碱对脂多糖诱导的人脐静脉内皮细胞炎症的影响[J].医药导报,2026,45(4):625-632,8.基金项目
国家重点研发计划资助项目(2018YFC1311300). （2018YFC1311300）
