中国南方果树2026，Vol.55Issue(1)：179-183,5.DOI:10.13938/j.issn.1007-1431.20240558
红阳猕猴桃核酮糖-1,5-双磷酸羧化酶/加氧酶小亚基基因的克隆与表达分析
Cloning and expression analysis of a Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit gene(AcRbcS)in Actinidia chinensis'Hongyang'
摘要
Abstract
Ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)plays a crucial role in plant photosynthesis,and its high expression is closely related to photosynthetic efficiency.Cloning the gene encoding the small subunit of ribulose-1,5-bi-sphosphate carboxylase/oxygenase(RuBisCo)in Hongyang kiwifruit(Actinidia chinensis'Hongyang'),and analyzing its expression pattern and biological function can provide a theoretical basis for targeted breeding strategies aimed at enhancing the photosynthetic productivity of Hongyang kiwifruit.The AcRbcS gene,a small subunit gene,was cloned from Hongyang kiwifruit leaf.Its expression profile in different leaf development stages was investigated using RNA-seq and real-time quantitative PCR(qPCR).The results showed that the full-length coding sequence(CDS)of AcRbcS was 369 bp,encoding a protein of 122 amino acids with a molecular weight of 13.75 kDa.The expression level of AcRbcS increased progressively during leaf development,reaching a peak at the light-green stage.This study provided valuable insights for future molecular breeding efforts to improve the photosynthetic efficiency and fruit quality of Hongyang kiwifruit.关键词
红阳猕猴桃/Rubisco小亚基/基因克隆/实时荧光定量PCRKey words
Actinidia chinensis'Hongyang'/RbcS/gene cloning/QPCR引用本文复制引用
何斌,刘仕丽,叶佳成,唐大海,刘林娅,黄亚成..红阳猕猴桃核酮糖-1,5-双磷酸羧化酶/加氧酶小亚基基因的克隆与表达分析[J].中国南方果树,2026,55(1):179-183,5.基金项目
贵州省科技厅项目(黔科合基础MS[2025]099) （黔科合基础MS[2025]099）
六盘水师范学院高层次人才科研启动基金项目(LPSSYKYJJ202205) （LPSSYKYJJ202205）
六盘水师范学院科研培育项目(LPSSY2023KJZDPY07) （LPSSY2023KJZDPY07）
贵州省教育厅自然科学研究项目(黔教技[2022]054号) （黔教技[2022]054号）
大学生创新创业训练计划项目资助(S202310977072) （S202310977072）
六盘水师范学院大学生科研训练计划项目(2024DK228)资助. （2024DK228）
