中国农业科技导报2026，Vol.28Issue(4)：57-64,8.DOI:10.13304/j.nykjdb.2024.0700
二倍体和四倍体南川百合差异性状的转录组分析
Transcriptome Analysis of Diffeential Taits Between Diploid and Tetrapllid Lilium rosthorii
摘要
Abstract
To investigate the changes of Lilium rosthorii homologous tetraploid with diploid,the contents of starch and soluble sugar in leaves of two kinds of ploidies of L.rosthorii were determined,and transcriptome sequencing analysis were performed.The results showed that the contents of soluble sugar and starch in leaves of the homologous tetraploid of L.rosthorii were significantly higher than those of the diploid,and the differences were significant with the extension of growth and development process.Compared with diploid,a total of 20 441 differentially expressed genes(DEGs)were found in tetraploid plants,among which 5 006 DEGs were annotated in GO(gene ontology)database,mainly enriched in plant growth,signal transduction regulation and other functions,and KEGG(Kyoto encyclopedia of gene and genomes)pathway analysis revealed significant differences in differential genes in pathways such as foreign substance metabolism,zeaxanthin synthesis and aminoacyl tRNA synthesis.In addition,7 DEGs related to glucose metabolism were screened and enriched in UDP glucosyltransferase activity and O-glycosyl compound hydrolase activity,and 10 DEGs related to pathogen response were screened and enriched in plant pathogen interactions and plant MAPK signaling pathway.Above results provided data foundation for in-depth research on gene function,also provided theoretical basis and data support for promoting the polyploidization process of lilies and cultivating excellent germplasm resources.关键词
南川百合/同源四倍体/转录组测序/差异表达基因Key words
Lilium rosthorii/homologous tetraploid/transcriptome sequencing/differentially expressed genes分类
农业科技引用本文复制引用
吕博妍,汪莲娟,符柳梅,高雪,贾桂霞..二倍体和四倍体南川百合差异性状的转录组分析[J].中国农业科技导报,2026,28(4):57-64,8.基金项目
河北省重点研发计划项目(21326317D) （21326317D）
国家重点研发计划项目(2019YFD1000400). （2019YFD1000400）
