中国药理学通报2026，Vol.42Issue(3)：558-565,8.DOI:10.12360/CPB202503089
基于网络药理学和实验验证探讨"黄芪-丹参-三七"角药对心衰大鼠氧化应激的影响
Effects of"Astragalus-Danshen-Sanqi"horn medicine on oxidative stress in rats with heart failure based on network pharmacology and experimental validation
摘要
Abstract
Aim To investigate the effects of Astragalus-Danshen-Sanqi horn medicine on oxidative stress in rats with heart failure model based on network pharmacology and experimental validation.Methods The drug active components,core targets and signal-ing pathways of Astragalus-Danshen-Sanqi horn medi-cine against oxidative stress in heart failure were screened by network pharmacology,and the core tar-gets were molecularly docked with the drug active com-ponents.Forty SD rats were randomly divided into four groups,the control group,model group,horn medi-cine group and western medicine group,and the corre-sponding drug interventions were given according to the subgroups.The changes in the level of cardiac function indexes in each group were compared using cardiac ultrasound,the pathological changes in car-diac tissues in each group were evaluated using HE staining,and the fibrosis of cardiomyocytes in each group was assessed using Masson staining;the changes in the ultrastructure of mitochondria of cardio-myocytes in each group were observed using transmis-sion electron microscopy;and the changes in the level of oxidative stress indexes in the cardiac tissues of rats in each group were compared.Results The key phar-macological components screened were quercetin,kaempferol,and lignocerotoxin,and the core targets of action were TNF,IL6,IL1B,TP53,AKT,and CASP3.Animal experiments showed that the cardiac function of rats was significantly improved when com-pared with that of the model group,and the results of HE and Masson staining showed that the structural damage of myocardial tissues was attenuated,the car-diomyocytes were less fibrous.Electron microscopy re-vealed reduced mitochondrial structure damage,while ROS levels in myocardial tissue were reduced,and CAT and T-AOC levels increased(P<0.05).Conclu-sion Horn medicine is able to exert a protective ef-fect against heart failure by reducing oxidative stress,mitigating mitochondrial structural and functional dam-age,and promoting the restoration of cardiac systolic-diastolic functions.关键词
黄芪-丹参-三七/角药/心力衰竭/氧化应激/网络药理学/线粒体Key words
Astragalus-Danshen-Sanqi/horn medi-cine/heart failure/oxidative stress/network pharma-cology/mitochondria分类
医药卫生引用本文复制引用
宋惠,杨斐斐,唐伟智,秦伟彬,张清伟,林琳,何贵新..基于网络药理学和实验验证探讨"黄芪-丹参-三七"角药对心衰大鼠氧化应激的影响[J].中国药理学通报,2026,42(3):558-565,8.基金项目
国家自然科学基金资助项目(No 81960861) （No 81960861）
国家中医药传承创新中心项目 （）
广西自然科学基金会重点项目(No 2020GXNSFDA297020) （No 2020GXNSFDA297020）
研究生教育创新计划项目(No YCBXJ2023033) （No YCBXJ2023033）
