中国药理学通报2026，Vol.42Issue(3)：584-592,9.DOI:10.12360/CPB202501023
基于RBMECs-PC12细胞共培养体系的缺糖缺氧/复氧损伤模型探讨当归-川芎药对活性成分脑保护作用机制
Study of brain protective mechanism of active ingredients in Danggui-Chuanxiong herb pair based on glucose deprivation hypoxia/reoxygenation injury model of RBMECs-PC12 cell coculture system
摘要
Abstract
Aim To explore the brain protective mechanism of the seven active ingredients(chloro-genic acid,ferulic acid,caffeic acid,butenylphtha-lein,senkyunolide H,senkyunolide A,ligustilide)in Danggui-Chuanxiong herb pair(GX).Methods An indirect co-culture method was used to construct a co-culture system of rat brain microvascular endothelial cells and adrenal pheochromocytoma cells(RBMECs-PC12).After 2,4,6,and 12 hours of glucose depri-vation and hypoxia,the system was reoxygenated for 24 hours.CCK-8 method was used to screen the opti-mal modeling time and administration concentration.The cells were divided into the control group,OGD/R group and low,medium and high dose groups of seven components.Flow cytometry was used to detect the apoptosis rate and ROS level in the co-culture system.RT-PCR was employed to detect the transcriptional lev-els of key genes of VEGF-AKT/NF-κB signaling path-way in the co-culture system.Results 12 hours of glucose deprivation and hypoxia in RBMECs-PC12 co-culture system was determined to be the best time for establishing the model.Compared with the OGD/R group,all the seven components could significantly re-duce the ROS level and the apoptosis rate of RBMECs-PC12 co-culture system.Chlorogenic acid,ferulic acid and caffeic acid significantly down-regulated the mRNA transcription levels of NF-κB p65 and MMP2.Chlorogenic acid and ferulic acid can also significantly down-regulate the mRNA transcription levels of Vegfr2 and Akt1.Senkyunolide H can up-regulate the mRNA transcription levels of Vegfr2 and Akt1.Conclusion The active components of Danggui-Chuanxiong herb pair can exert brain protection by reducing oxidative stress injury,reducing cell apoptosis rate,and inhibit-ing VEGF-AKT/NF-κB signaling pathway in RBMECs-PC12 co-culture system.关键词
当归-川芎药对/RBMECs-PC12细胞共培养体系/缺糖缺氧/复氧/活性成分/脑保护/作用机制Key words
Danggui-Chuanxiong herb pair/RBMECs-PC12 cell co-culture system/glucose depri-vation hypoxia/reoxygenation(OGD/R)/the active in-gredients/brain protection/action mechanism分类
医药卫生引用本文复制引用
孙文静,李锦燕,刘现磊,唐进法,纪秋如,李伟霞,王晓艳,张明亮,吴娅丽,杨柳青,孟伟亭,李旭阳..基于RBMECs-PC12细胞共培养体系的缺糖缺氧/复氧损伤模型探讨当归-川芎药对活性成分脑保护作用机制[J].中国药理学通报,2026,42(3):584-592,9.基金项目
国家自然科学基金资助项目(No 82004082,U1504827) （No 82004082,U1504827）
河南省科技研发计划联合基金(优势学科培育类)项目(No 222301420087) （优势学科培育类）
河南省中医药科学研究专项课题(No 2023ZY1003,2022ZY2010) （No 2023ZY1003,2022ZY2010）
河南省高校科技创新团队支持计划(No 23IRTSTHN026) （No 23IRTSTHN026）
河南省高校科技创新人才支持计划(No 25HASTIT061) （No 25HASTIT061）
