中国药理学通报2026，Vol.42Issue(3)：593-598,6.DOI:10.12360/CPB202505028
基于杂交瘤技术的BTN2A1单克隆抗体制备及其特异性研究
Preparation and specificity study of BTN2A1 monoclonal antibodies based on hybridoma technology
摘要
Abstract
Aim To explore the preparation method of mono-clonal antibodies targeting BTN2A1 and develop detection tools,providing experimental evidence for optimizing immuno-therapy strategies.Methods High-affinity BTN2A1 monoclo-nal antibodies were screened using hybridoma technology;anti-body specificity for native conformational antigens was validated by flow cytometry;epitope mapping was performed via competi-tive ELISA;feasibility of a dual-antibody sandwich detection system was assessed based on antibody pairing experiments.Re-sults Fifteen high-affinity BTN2A1 monoclonal antibodies were successfully obtained(minimum half-maximal effective concentration:0.002 862 g·L-1).Flow cytometry confirmed that all antibodies specifically recognized cell-surface native BTN2A1 in BTN2A1-overexpressing CHO cells and Daudi tumor cell lines with>95%binding positivity.Epitope analysis indicated all antibodies targeted the same epitope.Conclu-sions This study successfully generates a panel of high-affinity BTN2A1 monoclonal antibodies,providing essential tools for functional research.However,since all antibodies recognize an identical epitope,establishing a dual-antibody sandwich detec-tion system is unfeasible.Further development of antibody com-binations targeting distinct epitopes is thus required,laying the foundation for optimizing BTN2A1 immunoassays and therapeu-tic antibody development.关键词
Vγ9Vδ2T细胞/BTN2A1/单克隆抗体/T细胞/抗体/免疫Key words
Vγ9Vδ2 T cells/BTN2A1/monoclonal antibody/T cells/antibody/immunity分类
医药卫生引用本文复制引用
伍靳函,牛亚燕,蔡宁宁,吴妹英..基于杂交瘤技术的BTN2A1单克隆抗体制备及其特异性研究[J].中国药理学通报,2026,42(3):593-598,6.基金项目
国家自然科学基金资助项目(No 82400008) （No 82400008）
国家重点研发计划(No 2023YFC2307300) （No 2023YFC2307300）
苏州市呼吸道感染性疾病临床医学中心(No Szlcyxzx202108) （No Szlcyxzx202108）
江苏省血地寄防科研项目(No x202346) （No x202346）
