中国实验动物学报2026，Vol.34Issue(3)：362-370,9.DOI:10.3969/j.issn.1005-4847.2026.03.005
基于16S rDNA测序的中国地鼠和金黄地鼠的肺部和肠道微生物群比较
Comparison of microbiota in Chinese hamsters and golden hamsters based on 16S rDNA sequencing
摘要
Abstract
Objective To compare the composition and diversity of lung and gut microbiota between Chinese and golden hamsters using 16S rDNA sequencing,to provide a new perspective for understanding their applications in human disease research.Methods SPF-grade Chinese hamsters and golden hamsters(6 males and 6 females per group)aged 6～8 weeks were selected.Bronchoalveolar lavage fluid,small intestinal contents,and large intestinal contents were collected under sterile conditions.Species annotation and α-diversity and β-diversity analyses were performed using 16S rDNA sequencing.Linear discriminant analysis effect size(LEfSe)analysis was employed to identify potential biomarkers.Results The α-diversity of the lung and small intestinal microbiota was significantly higher in golden hamsters(especially males)than in Chinese hamsters(P＜0.05),while the diversity of the large intestinal microbiota was significantly higher in Chinese hamsters(P＜0.05).Regarding β-diversity,the composition of the lung,small intestinal,and large intestinal microbiota differed significantly between the two types of hamsters(P＜0.01).LEfSe analysis revealed differential occurrence of the phylum Firmicutes in the lung microbiota in golden hamsters,and differential occurrence of the phyla Bacteroidota(females)and Proteobacteria(males)in Chinese hamsters.Regarding the small intestinal and large intestinal microbiota,Firmicutes and Bacteroidota were differential phyla in Chinese and golden hamsters,respectively.Conclusions The composition and diversity of the lung and gut microbiota differed significantly between Chinese and golden hamsters,which may be related to their distinct applications in human disease research.This study provides a new perspective for understanding the applications of these two types of hamsters in human disease research and lays a theoretical foundation for future efforts to improve their health status and their value as experimental models by modulating their microbiota.关键词
中国地鼠/金黄地鼠/16S rDNA测序/微生物群Key words
Chinese hamster/golden hamster/16S rDNA sequencing/microbiota分类
生物科学引用本文复制引用
刘凯慧,郭亚茜,杜晓鹏,王昭华,朱华..基于16S rDNA测序的中国地鼠和金黄地鼠的肺部和肠道微生物群比较[J].中国实验动物学报,2026,34(3):362-370,9.基金项目
国家重大协同创新项目(2022-I2M-1-020).Funded by the National Major Collaborative Innovation Project(2022-I2M-1-020). （2022-I2M-1-020）
