中兽医医药杂志2026，Vol.45Issue(2)：1-8,8.DOI:10.13823/j.cnki.jtcvm.2026.005
甘草发酵前后活性成分变化及抗氧化作用研究
Analysis of changes in active components and antioxidant activity of Radix Glycyrrhizae before and after fermentation
摘要
Abstract
Radix Glycyrrhizae was fermented using Bacillus velezensis to compare the changes in active components and antioxidant effects before and after fermentation.Spectrophotometry and high-performance liquid chromatography(HPLC)were employed for quantitative analysis,while ABTS+·,·O2-and DPPH·radical scavenging assays were used to evaluate the antioxidant activity of Radix Glycyrrhizae extracts before and after fermentation.The results showed that the contents of total flavonoids(1.405 mg/mL),total polyphenols(1.037 mg/mL),and total saponins(8.821 mg/mL)in fermented Radix Glycyrrhizae extracts were significantly higher(P<0.05)than those before fermentation(0.647 mg/mL,0.404 mg/mL,and 6.864 mg/mL,respectively).HPLC analysis revealed that the contents of liquiritin apioside,isoliquiritin apioside,liquiritigenin,and glycyrrhizic acid were all significantly higher(P<0.05)after fermentation,with glycyrrhizic acid content increasing to 2.66 times of that before fermentation.In contrast,the contents of liquiritin and isoliquiritin decreased relative to their pre-fermentation levels.The scavenging rates of the fermented extract on ABTS+·and·O2-increased by 83.60%and 39.56%,respectively,compared to those before fermentation,indicating a significant enhancement in scavenging efficacy.However,the DPPH·radical scavenging rate decreased by 25.43%compared to that before fermentation.In conclusion,fermentation significantly altered the content of active components in Radix Glycyrrhizae and improved its antioxidant effects.关键词
甘草/发酵/活性成分/抗氧化活性Key words
Radix Glycyrrhizae/fermentation/active components/antioxidant activity分类
农业科技引用本文复制引用
郑雪,高海宁,张东芝,尹珊,张勇,李彩霞..甘草发酵前后活性成分变化及抗氧化作用研究[J].中兽医医药杂志,2026,45(2):1-8,8.基金项目
甘肃省科技计划项目(22JR5RG564) （22JR5RG564）
甘肃省自然科学基金项目(20JR10RG299) （20JR10RG299）
全国大学生创新创业计划训练项目(202210740063,202210740010,S202310740023) （202210740063,202210740010,S202310740023）
