中国实验动物学报2026，Vol.34Issue(3)：424-431,8.DOI:10.3969/j.issn.1005-4847.2026.03.010
脓毒症小鼠模型制备及免疫抑制状态评价的研究进展
Research progress on the establishment of septic mouse models and evaluation of immunosuppressive status
摘要
Abstract
Sepsis is a life-threatening condition associated with high mortality rates in clinical settings.The subsequent immunosuppressive state is strongly correlated with adverse patient outcomes.Establishing robust animal models is therefore essential for elucidating the pathophysiological mechanisms of sepsis-induced immunosuppression and developing therapeutic interventions.Current models,however,are predominantly employed in sepsis hyperinflammation studies,while their utilization specifically for the immunosuppression phase remains limited.In addition,standardized assessment criteria for immunosuppression are lacking across different investigations.Commonly employed models include cecal ligation and puncture(CLP)and lipopolysaccharide(LPS)administration.Although cecal ligation and puncture effectively recapitulates the clinical pathology,it suffers from significant outcome variability.Conversely,although lipopolysaccharide administration is technically straightforward,its artificial nature fails to replicate the polymicrobial pathogenesis of natural infection.Quantitative analysis of immune cell populations,cytokine profiles,and immune organ status provides a reliable assessment of immunosuppression severity in animal models.By synthesizing recent literature,this review consolidates the method ologies and assessment parameters for establishing immunosuppressive sepsis models.By critically evaluating the strengths and limitations of each approach,the review aims to establish a standardized experimental framework for the future development of optimized sepsis models.关键词
脓毒症/免疫抑制/动物模型/评估指标Key words
sepsis/immunosuppression/animal model/evaluation indicator分类
生物科学引用本文复制引用
李昌奇,李昕洋,陈波,张靖宇,王婧文,匡小冰,李柠岑,傅丽媛,马培宏..脓毒症小鼠模型制备及免疫抑制状态评价的研究进展[J].中国实验动物学报,2026,34(3):424-431,8.基金项目
国家自然科学基金(82575215,82374563),天津市科技计划项目(25YDTPJC00430).Funded by National Natural Science Foundation of China(82575215,82374563),Tianjin Science and Technology Program Project(25YDTPJC00430). （82575215,82374563）
