中兽医医药杂志2026，Vol.45Issue(2)：17-22,6.DOI:10.13823/j.cnki.jtcvm.2026.004
31种中药提取液对猪链球菌体外抑菌活性及复方筛选研究
The antibacterial activity of 31 Chinese herbal medicine extracts against Streptococcus suis in vitro and screening of their compound formulations
摘要
Abstract
The purpose of this experiment is to screen compound Chinese medicines with good antibacterial effects on Streptococcus suis. 31 traditional Chinese medicines were extracted using distillation and extraction methods,respectively. The Oxford cup method and double dilution method were used to determine the in vitro antibacterial zone size,minimum inhibitory concentration (MIC),and minimum bactericidal concentration (MBC) values of each traditional Chinese medicine extract against Streptococcus suis. The four traditional Chinese medicines with the strongest antibacterial effect were selected and compounded in different combinations in a 1:1 ratio. The MIC and MBC values of each group of traditional Chinese medicines after compounding were measured to screen for the best anti Streptococcus suis traditional Chinese medicine compound combination. The results showed that among the 31 traditional Chinese medicine extracts and distillates tested,18 had antibacterial effects on Streptococcus suis. In the inhibition zone test,the diameters of the inhibition zones of Sappan Lignum,Coptidis Rhizoma,Chebulae Fructus,Galla Chinensis,Phellodendri Chinensis Cortex,Allii Sativi Bulbus,Scutellariae Radix extract,and Cinnamomi Cortex distillate were all greater than 20 mm,indicating high sensitivity. In the MIC and MBC determination experiments,the MIC and MBC of the extracts of Galla Chinensis,Coptidis Rhizoma,Sappan Lignum,Phellodendri Chinensis Cortex were 0.49 mg/mL and 0.98 mg/mL,0.98 mg/mL and0.98 mg/mL,0.98 mg/mL and 1.95 mg/mL,and 1.95 mg/mL and 3.90 mg/mL,respectively,which were lower than those of other treatment groups. The extracts of these 4 Chinese herbal medicines were grouped into 11 combinations and compounded. After compounding,the MIC and MBC of the combination of Sappan Lignum and Galla Chinensis was both0.24 mg/mL,which was lower than those of other treatment groups. The results of the in vitro antibacterial test showed that among the 31 medicinal herbs,the single herb of Sappan Lignum,Coptidis Rhizoma,Phellodendri Chinensis Cortex,and Galla Chinensis present the best in vitro antibacterial effect on Streptococcus suis. In the compound combination,the combination of Sappan Lignum and Galla Chinensis has the strongest in vitro antibacterial activity with the lowest MIC and MBC values.关键词
中药/复方组合/猪链球菌/蒸馏提取/浸提Key words
traditional Chinese medicine/compound combination/Streptococcus suis/distillation extraction/extraction分类
农业科技引用本文复制引用
赵聪,赵霞,黄斌斌,许新春,康朝勇,孙小帅,杜家军,岳治光..31种中药提取液对猪链球菌体外抑菌活性及复方筛选研究[J].中兽医医药杂志,2026,45(2):17-22,6.基金项目
河南省重点研发专项(231111111600) （231111111600）
