中国沼气2026，Vol.44Issue(2)：21-29,9.DOI:10.20022/j.cnki.1000-1166.2026020021
黑曲霉发酵餐厨垃圾三相固渣产酶的性能研究
Enzyme Production Performance by Aspergillus niger Fermentation of Three-phase Solid Residue from Food Waste
摘要
Abstract
Commercial enzymes have garnered widespread attention in enhancing the treatment performance of organic solid waste.However,their high raw material and sterilization costs restrict large-scale applications.In this study,three-phase solid residue from food waste,obtained through wet heat hydrolysis and phase separation of food waste slurry,was used as a low-cost,non-sterilized fermentation substrate for the production of composite enzymes by Aspergillus niger.The results showed that A.niger could effectively grow on the three-phase solid residue and produce amylase,protease,cellulase,and lipase.Under a pH of 5.5,the activity of acidic protease,cellulase,and lipase was optimal,while amylase activity peaked at pH 6.5.Orthogonal experiments indicated that pH had the greatest effect on enzyme activity,followed by the inoculum size and fermentation time.The optimal fermentation conditions,determined by the efficacy coefficient method combined with AHP-CRITIC mixed weighting analysis,were pH 5.5,inoculum volume of 2 mL(Corresponding to 30 g three-phase solid residue),and a cultivation time of 6 days.The findings are expected to further promote the application of low-cost en-zyme preparations in the organic solid waste field and provide a new perspective for valorization of food waste three-phase solid residue.关键词
餐厨垃圾/三相固渣/黑曲霉/固态发酵/酶活Key words
food waste/three-phase solid residue/Aspergillus niger/solid-state fermentation/enzyme activity分类
农业科技引用本文复制引用
张瑞华,王勇群,徐恒,刘晓吉,刘青,谷艳芳,尚斯源,张莹莹,王志华,罗娟,刘秋琳..黑曲霉发酵餐厨垃圾三相固渣产酶的性能研究[J].中国沼气,2026,44(2):21-29,9.基金项目
合肥市关键共性技术研发"揭榜挂帅"项目(GJ2022SH12) （GJ2022SH12）
中节能(肥西)环保能源有限公司科技创新项目(Q/ZH3-SC011-A/0-2021). （肥西）
