中兽医医药杂志2026，Vol.45Issue(2)：44-50,7.DOI:10.13823/j.cnki.jtcvm.2026.002
鸡血浆中连翘脂素UPLC-MS/MS检测方法的建立
Development and validation of an UPLC-MS/MS method for the quantification of phillygenin in chicken plasma
摘要
Abstract
This study aimed to develop an ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for the accurate determination of phillygenin in chicken plasma.Chromatographic separation was performed using an Agilent Poroshell 120 EC-C18 column(100.0 mm×4.6 mm,2.7 µm)with a gradient elution program consisting of methanol and 0.1%formic acid solution as the mobile phase.Mass spectrometric detection was carried out in negative ion mode using multiple reaction monitoring(MRM).The method demonstrated good linearity over the concentration range of 10-1 280 ng/mL(R²>0.99).The intra-day and inter-day precision,expressed as relative standard deviation(RSD),were both below 15%,with accuracy ranging from 92.25%to 106.93%.The mean extraction recovery was between 86.90%and 99.28%,and matrix effects ranged from 89.24%to 104.72%.The samples also showed good stability under all tested storage conditions.The developed method exhibited high specificity,accuracy,and reliable performance,making it suitable for the quantitative analysis of phillygenin in chicken plasma.关键词
连翘脂素/超高效液相色谱-串联质谱法/鸡血浆Key words
phillygenin/UPLC-MS/MS/chicken plasma分类
农业科技引用本文复制引用
孟冰冰,张康,徐国伟,王磊,郭志廷,刘淑琪,古雪艳,李建喜,张景艳..鸡血浆中连翘脂素UPLC-MS/MS检测方法的建立[J].中兽医医药杂志,2026,45(2):44-50,7.基金项目
"十四五"国家重点研发计划项目(2023YFD1800804-02) （2023YFD1800804-02）
中国农业科学院科技创新工程中兽医与临床团队建设项目(CAAS-ASTIP-2015-LIHPS) （CAAS-ASTIP-2015-LIHPS）
