中兽医医药杂志2026，Vol.45Issue(2)：51-56,6.DOI:10.13823/j.cnki.jtcvm.2026.008
泰山磐石散加减方体外抗氧化活性评价
Evaluation of the antioxidant activity of Taishan Panshi San modified formula in vitro
摘要
Abstract
To evaluate the antioxidant activity of the modified Taishan Panshi San formula in vitro,four classical models(DPPH·,ABTS+·,·OH and FRAP)were employed,with vitamin C(VC)acting as the positive control,to determine its capacity to scavenge DPPH·,ABTS+·,·OH radicals and reduce Fe3+(FRAP assay).Absorbance was measured at 517 nm,734 nm,520 nm and 593 nm,respectively,and the scavenging rates were calculated.Fe3+reduction capacity was determined via a FeSO4 standard curve.The results showed that the scavenging rates of the modified Taishan Panshi San formula against all three free radicals increased with concentration,exhibiting a clear dose-response relationship.The IC50 values were 0.274 5 mg/mL for DPPH·radicals,0.153 1 mg/mL for ABTS+·radicals and 0.526 7 mg/mL for·OH,all of which were lower than the IC50 value of vitamin C(0.002 1 mg/mL,0.011 3 mg/mL and 0.024 9 mg/mL,respectively).The Fe3+reduction capacity of the modified Taishan Panshi San formula exhibited a positive correlation,increasing with formula concentration,while Fe3+reduction capacity of the formulation(0.059 5 mmol FeSO4/g)was significantly lower than that of vitamin C(100.830 8 mmol FeSO4/g).Taishan Panshi San demonstrates scavenging activity against DPPH·,ABTS+·and hydroxyl radicals,as well as iron ion reduction capacity.关键词
泰山磐石散加减方/抗氧化活性/自由基清除活性/Fe3+还原能力Key words
Taishan Panshi San/antioxidant activity/radicals scavenging activity/Fe3+reduction capacity分类
农业科技引用本文复制引用
廖心怡,郝健均,付涵,李如怡,蔡远花,李桐阳,王语嫣,买占海..泰山磐石散加减方体外抗氧化活性评价[J].中兽医医药杂志,2026,45(2):51-56,6.基金项目
2025年新疆农业大学校级大学生创新训练计划项目(dxscx2025105) （dxscx2025105）
