中兽医医药杂志2026，Vol.45Issue(2)：57-62,6.DOI:10.13823/j.cnki.jtcvm.2026.009
5种解热镇痛药中违规添加马来酸氯苯那敏的HPLC-PDA检测方法研究
Determination of chlorphenairmine maleate illegal adulterated into 5 antipyretic analgesics by HPLC-PDA
摘要
Abstract
The objective was to establish a method for the detection of illegal addition of chlorpheniramine maleate in 5 kinds of antipyretic analgesics.Eicoee WT C18(4.6 mm×150.0 mm,5µm)was used.The mobile phase was 0.02 mol/L sodium heptanesulfonate solution(containing 1%triethylamine,adjusted to pH 5.5 with phosphoric acid)-acetonitrile(70∶30,V∶V);the flow rate was 1.0 mL/min.PDA detector,scanning range of 200-400 nm,detection wavelength was 260 nm,column temperature was 30℃,and the injection volume was 10µL.The linear range of chlorphenamine maleate was 0.052-41.500 µg/mL(r=0.999 97).The average recoveries of analgin injection,aminopyrine injection,antonidine injection,paracetamol injection and diclofenac sodium injection were 100.7%(RSD=0.9%),98.1%(RSD=0.6%),98.6%(RSD=0.4%),97.8%(RSD=0.9%)and 99.1%(RSD=0.6%),respectively.The method has good resolution,high sensitivity,simplicity and accuracy,and can be used for the detection of the content of chlorpheniramine maleate illegally added in common antipyretic analgesic injection.关键词
马来酸氯苯那敏/解热镇痛药/非法添加/HPLCKey words
chlorpheniramine maleate/antipyretic analgesics/illegal addition/HPLC分类
农业科技引用本文复制引用
邱天宝,贾楠楠,刘占通,朱雷,董鹏,李慧素,赵富华..5种解热镇痛药中违规添加马来酸氯苯那敏的HPLC-PDA检测方法研究[J].中兽医医药杂志,2026,45(2):57-62,6.基金项目
河南省市场监督管理局科技计划项目(HNKE2025031314860027034145) （HNKE2025031314860027034145）
