中兽医医药杂志2026，Vol.45Issue(2)：73-77,5.DOI:10.13823/j.cnki.jtcvm.2026.015
兽用复方酮康唑软膏含量测定方法的改进
Improvement of the content determination method for veterinary compound ketoconazole ointment
许晓靖 1吴昊 1许世富 1刘红云 1吴蕾 1陈文帮 1单晨芳1
作者信息
- 1. 安徽省兽药饲料监察所,安徽 合肥 230000
- 折叠
摘要
Abstract
A high-performance liquid chromatography method was established and validated for the simultaneous determination of ketoconazole and metronidazole in compound ketoconazole veterinary preparations.An octadecylsilane-bonded silica gel column(Waters XSelect CSH C18,4.6 mm×250.0 mm,5 µm)was employed,with methanol-water(74∶26,V/V)as the mobile phase.The flow rate was set at 1.0 mL/min,the column temperature was controlled at 30℃,and the detection wavelength was 239 nm.The experimental results demonstrated that ketoconazole showed a good linear relationship within the concentration range of 0.16-0.64 mg/mL(R2=0.999 3),and metronidazole also exhibited favorable linearity in the range of 0.32-1.12 mg/mL(R2=0.999 5).The average recoveries of ketoconazole and metronidazole were 100.2%and 99.8%,respectively,with the relative standard deviations(RSDs)of 1.12%and 0.94%.The current method for this product is recorded in Veterinary Drug Quality Standards(2017 Edition),Volume of Chemical Drugs.Nevertheless,it is time-consuming in practical operation,and the reagents involved pose certain hazards to human beings and the environment.The method developed in this study can significantly improve the experimental efficiency,effectively reduce pollution and related hazards,thus provide solid technical support for the revision and upgrading of the quality standard of this veterinary drug.关键词
酮康唑/甲硝唑/含量测定/高效液相色谱法Key words
ketoconazole/metronidazole/content determination/high performance liquid chromatography(HPLC)分类
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许晓靖,吴昊,许世富,刘红云,吴蕾,陈文帮,单晨芳..兽用复方酮康唑软膏含量测定方法的改进[J].中兽医医药杂志,2026,45(2):73-77,5.
