中药与临床2026，Vol.17Issue(2)：1-6,6.
冬虫夏草多糖提取方法比较与优化
Comparative study on extraction methods and process optimization of Cordyceps polysaccharides
摘要
Abstract
Objective:To improve the extraction yield of Cordyceps polysaccharides and optimize the extraction process of Cordyceps polysaccharides.Methods:Cordyceps polysaccharides were extracted using ultrasound-assisted extraction and internal boiling method,and the effects of these two methods on polysaccharide yield were compared through single-factor experiments.The optimal method was selected for further Box-Behnken response surface methodology to optimize the extraction process.Re-sults:The ultrasonic-assisted extraction method outperformed the internal boiling method,with extraction rates of 2.718％and 2.058％,respectively.Through response surface analysis,the optimal extraction conditions for Cordyceps polysaccharides were determined as follows:ultrasonic temperature set at 55℃,ultrasonic power adjusted to 280 W,ultrasonic treatment time of 30 min,and a liquid-to-material ratio of 30.Three replicate experiments were conducted under these conditions to verify the reliabil-ity of the optimized process,resulting in an average polysaccharide extraction yield of 2.714％.Conclusion:The optimized extrac-tion process is efficient,time-saving,and conducive to the further development and utilization of natural Cordyceps.关键词
冬虫夏草/多糖/超声波辅助提取法/内部沸腾法/响应面Key words
Cordyceps/polysaccharides/ultrasound-assisted method/internal boiling method/response surface分类
医药卫生引用本文复制引用
白静,杨兴茂,李勇,王平,田文静,国锦琳..冬虫夏草多糖提取方法比较与优化[J].中药与临床,2026,17(2):1-6,6.基金项目
国家自然科学基金(82373998) （82373998）
