中国动物检疫2026，Vol.43Issue(3)：14-22,9.DOI:10.3969/j.issn.1005-944X.2026.03.003
新疆喀什地区鸽A群轮状病毒核酸检测及遗传进化分析
Nucleic Acid Detection and Genetic Evolution Analysis of Pigeon Rotavirus A in Kashgar Prefecture,Xinjiang
摘要
Abstract
In order to investigate the prevalence and genetic evolutionary characteristics of group A rotavirus(RVA)in pigeons in Kashgar Prefecture,Xinjiang,165 pigeon swab samples were collected from 9 farms in the region in 2024 to detect RVA nucleic acid using real-time RT-PCR.8 representative positive samples were selected for RT-PCR amplification and sequencing targeting the two neutralization genes,VP7 and VP4,followed by analysis of nucleotide homology and genetic evolution.The results showed that 16 out of 165 swab samples from 8 farms were tested positive for RVA,with an individual positivity rate of 9.70％(16/165).Genetic evolution analysis based on the VP7 and VP4 genes of representative positive samples revealed that all the 8 strains belonged to genotype G18P[17].The nucleotide homology of VP7 ranged from 85.2％to 99.9％,and from 58.3％to 98.7％compare with 18 reference strains,while that of VP4 was from 89.5％to 99.7％,and from 52.8％to 95.8％compare with 18 reference strains.All the positive representative samples including XJ-92,XJ-63,XJ-110 and XJ-01 were highly and genetically related to the US pigeon-derived strain WVL21015-FL,which were speculated to have originated from the same outbreak or source.XJ-71 was more distantly related to the other representative positive samples,with the homology from 89.5％to 91.4％,but remained the closest to the Australian strain VIC,with the homology of 88.6％.In conclusion,RVA infection was seriously and widely prevalent in the region,dominated by G18P[17];the RVA strains prevalent in pigeon populations in Xinjiang were classified into the same evolutionary branch or originated from the same source,and gene mutations or genomic reassortment might occur during their transmission.The prevalence and transmission of pigeon RVA were recommended to control through continuously strengthening RVA surveillance,improving good practices on farms and accelerating the development of RVA vaccines.关键词
鸽A群轮状病毒/流行病学调查/VP4基因/VP7基因/核酸检测/遗传进化分析Key words
RVA/epidemiological investigation/VP4 gene/VP7 gene/nucleic acid test/genetic evolution analysis分类
农业科技引用本文复制引用
陈雨腾,李阳,闫昊,于晓慧,王碧政,陈璐,刘玉峰,张翠瑶,张苗苗,李金平,王静静,刘华雷..新疆喀什地区鸽A群轮状病毒核酸检测及遗传进化分析[J].中国动物检疫,2026,43(3):14-22,9.基金项目
2023年黄河流域协同科技创新(科技对口支援和东西部协作)项目(YDZX2023009) （科技对口支援和东西部协作）
