中医药学报2026，Vol.54Issue(4)：31-38,8.DOI:10.19664/j.cnki.1002-2392.260072
紫花地丁HPLC指纹图谱及其抗炎作用研究
Study on HPLC Fingerprint and Anti-inflammatory Effects of Viola yedoensis
摘要
Abstract
Objective:To establish an HPLC fingerprint of Viola yedoensis using HPLC technology,evaluate the quality of 10 batches of Viola yedoensis combined with cluster analysis(HCA)and principal component analysis(PCA),and study its anti-inflammatory mechanism.Methods:A Waters Symmetry C18 column(250 mm×4.6 mm,5 μm)was used with a mobile phase of acetonitrile-0.1%phosphoric acid aqueous solution;flow rate:0.8 mL·min-1;injection volume:10 μL;column temperature:30℃;detection wavelength:353 nm.The anti-inflammatory effect was observed using the ear swelling method,and the levels of PGE2,IL-6,and TNF-α in serum were detected.Results:The HPLC fingerprint of 10 batches of Viola yedoensis was successfully established,with 10 common peaks identified.The similarities were all higher than 0.990.Two common peaks were identified as cichoriin(2.44±1.11)mg·g-1 and esculetin(10.50±1.57)mg·g-1.The 10 batches of samples were classified into two categories by chemical pattern recognition methods.The aqueous decoction of Viola yedoensis could inhibit ear swelling in mice and reduce the levels of TNF-α,IL-6,and PGE2 in serum.Conclusion:This study successfully established the HPLC fingerprint of 10 batches of Viola yedoensis and found that its anti-inflammatory mechanism may be related to the inhibition of TNF-α,IL-6,and PGE2 expression,providing a scientific basis and pharmacodynamic support for the quality control and clinical application of Viola yedoensis.关键词
紫花地丁/指纹图谱/化学模式识别/抗炎/质量评价Key words
Viola yedoensis/Fingerprint/Chemical pattern recognition/Anti-inflammatory/Quality evaluation分类
医药卫生引用本文复制引用
孙杭,张振华,黄莉莉..紫花地丁HPLC指纹图谱及其抗炎作用研究[J].中医药学报,2026,54(4):31-38,8.基金项目
黑龙江省自然科学基金项目(LH2019H106) （LH2019H106）
黑龙江省省属本科高校"优秀青年教师基础研究支持计划"(YQJH2023152) （YQJH2023152）
