中国药房2026，Vol.37Issue(7)：877-882,6.DOI:10.6039/j.issn.1001-0408.2026.07.09
基于GRB2/ERK/CRLS1通路探讨益肺宣肺降浊方对血管性痴呆模型大鼠的改善作用机制
Study on the improving mechanism of Yifei xuanfei jiangzhuo formula on vascular dementia model rats based on the GRB2/ERK/CRLS1 pathway
摘要
Abstract
OBJECTIVE To explore the improvine mechanism of Yifei xuanfei jiangzhuo formula(YFXF)on vascular dementia(VAD)model rats based on the growth factor receptor-bound protein 2(GRB2)/extracellular signal-regulated kinase(ERK)/cardiolipin synthase 1(CRLS1)pathway.METHODS VAD rat model was established by permanent bilateral common carotid artery ligation.Forty-eight successfully modeled rats were randomly divided into the model group(normal saline),donepezil hydrochloride group(positive control group,0.2 g/kg),and YFXF low-and high-dose groups(12.18 and 24.36 g/kg,calculated based on the total amount of crude drug),respectively.In addition,a sham operation group(normal saline)was set up.There were 12 rats in each group.Daily intragastric administration of drug or normal saline was performed for 30 consecutive days.After the last administration,the spatial cognitive ability of the rats was evaluated,the pathological morphology of the hippocampus was observed,the contents of tumor necrosis factor-α(TNF-α)and interleukin-4(IL-4)in serum were detected,the expression levels of GRB2/ERK/CRLS1 pathway-related proteins and the mRNA levels of GRB2,CRLS1,NADH dehydrogenase subunit 1(ND1),Tafazzin(TAZ),phospholipid scramblase 3(PLSCR3)and the ATP content in hippocampal tissue were measured.RESULTS Compared with the sham operation group,the escape latency of rats in the model group was significantly prolonged(P＜0.05),and the number of crossing platform was significantly reduced(P＜0.05),while the number of pyramidal cells and Nissl bodies in the hippocampus decreased sharply;the content of TNF-α in serum was significantly increased(P＜0.05),and the content of IL-4 was significantly decreased(P＜0.05);the expression levels of GRB2 and CRLS1 proteins,the phosphorylation level of ERK protein,the relative expression levels of GRB2,CRLS1,ND1,TAZ,and PLSCR3 mRNA,and the content of ATP in hippocampal tissue were significantly decreased(P＜0.05).Compared with the model group,the above pathological changes in the hippocampal tissue of each administration group were alleviated,and the quantitative indicators were significantly restored(P＜0.05).CONCLUSIONS YFXF may improve hippocampal neuron injury in VAD rats by activating the GRB2/ERK/CRLS1 pathway,maintaining cardiolipin homeostasis,and improving mitochondrial energy metabolism.关键词
益肺宣肺降浊方/血管性痴呆/神经元损伤/GRB2/ERK/CRLS1通路/线粒体/心磷脂Key words
Yifei xuanfei jiangzhuo formula/vascular dementia/neuron injury/GRB2/ERK/CRLS1 pathway/mitochondria/cardiolipin分类
医药卫生引用本文复制引用
卓桂锋,陈炜,朱小敏,符钰岚,张金枝,吴林..基于GRB2/ERK/CRLS1通路探讨益肺宣肺降浊方对血管性痴呆模型大鼠的改善作用机制[J].中国药房,2026,37(7):877-882,6.基金项目
国家自然科学基金项目(No.82374387) （No.82374387）
广西自然科学基金项目(No.2025GXNSFAA069990) （No.2025GXNSFAA069990）
广西中医药大学引进博士科研启动基金项目(No.2024BS012) （No.2024BS012）
