中医临床研究2026，Vol.18Issue(1)：22-27,6.DOI:10.3969/j.issn.1674-7860.2026.01.003
基于丝裂原活化蛋白激酶信号通路探讨黄芪多糖对肠上皮-巨噬细胞共培养肠炎模型的改善作用及机制
Exploring the therapeutic effect and mechanism of astragalus polysaccharides on intestinal epithelial cell-macrophage co-cultured enteritis model via the MAPK signaling pathway
摘要
Abstract
Objective:To investigate the ameliorative effect of astragalus polysaccharide(APS)on the inflammatory response in the Caco-2/RAW264.7 co-culture intestinal model and explore its potential mechanism.Methods:Different concentrations of APS(50 μg/mL,100 μg/mL)were applied to the Caco-2/RAW264.7 co-culture intestinal inflammation model induced by Lipopolysaccharide(LPS).The protective effect of APS on intestinal barrier function damage was evaluated by measuring the activities of intestinal alkaline phosphatase(IAP)and diamine oxidase(DAO),and the expression of tight junction protein zonula occludens-1(ZO-1).Meanwhile,the potential molecular mechanism of APS in improving intestinal inflammation was explored by determining the levels of inflammatory cytokines(TNF-α,IL-1β,IL-6)and the phosphorylation levels of ERK and p38 MAPK proteins in the mitogen-activated protein kinase(MAPK)signaling pathway.Results:Compared with the model group,the activities of IAP and DAO in the high-dose group(100 μg/mL)were statistically significantly increased(P<0.05).The expression of ZO-1 protein in both the high-dose and low-dose groups(100 μg/mL,50 μg/mL)were statistically significantly increased in a dose-dependent manner(P<0.05).In addition,the secretion of inflammatory cytokines TNF-α,IL-1β,and IL-6 in the high-dose group were statistically significantly reduced(P<0.05).Both the high-dose and low-dose groups significantly inhibited the activation of the MAPK signaling pathway and decreased the phosphorylation levels of ERK and p38 MAPK proteins,thereby effectively suppressing the inflammatory response(P<0.05).Conclusion:APS can alleviate the inflammatory response in the intestinal co-culture model by inhibiting the MAPK signaling pathway and protect the integrity of the intestinal barrier,which provides a theoretical basis for the application of APS in the development of natural anti-inflammatory foods and drugs.关键词
黄芪多糖/Caco-2/RAW264.7共培养肠炎模型/肠道屏障/炎症/丝裂原活化蛋白激酶信号通路Key words
Astragalus polysaccharide/Caco-2/RAW264.7 cell co-culture model/Intestinal barrier/Inflammation/MAPK signaling pathway分类
医药卫生引用本文复制引用
钟晞,慕嘉欣,许晓蕾,吴莉..基于丝裂原活化蛋白激酶信号通路探讨黄芪多糖对肠上皮-巨噬细胞共培养肠炎模型的改善作用及机制[J].中医临床研究,2026,18(1):22-27,6.基金项目
江西省卫生健康委科技计划(202211406) （202211406）
江西省研究生创新专项(YC2024-B220) （YC2024-B220）
江西中医药大学大学生创新创业训练计划(202410412155X、202410412149). （202410412155X、202410412149）
