中国药房2026，Vol.37Issue(7)：908-913,6.DOI:10.6039/j.issn.1001-0408.2026.07.14
竹节参皂苷Ⅳa对糖尿病肾病大鼠的肾脏保护作用及机制
Protective effect and mechanism of chikusetsu saponin Ⅳa on the kidney in diabetic nephropathy rats
摘要
Abstract
OBJECTIVE To study the protective effect and potential mechanism of chikusetsu saponin Ⅳa(chsⅣ)on renal function in diabetic nephropathy(DN)model rats.METHODS DN rat model was established by high-fat diet combined with streptozotocin injection.Thirty-six model rats were randomly divided into model group(i.g.administration of normal saline,high-fat diet),chsⅣ low-dose and high-dose groups(i.g.administration of 90,180 mg/kg chsⅣ,high-fat diet),with 12 rats in each group.Additionally,10 normal rats were set as the control group(i.g.administration of normal saline,regular diet).From the 5th to the 12th week after streptozotocin injection,they were given intragastric administration of relevant drug or normal saline,once a day.After the last medication,the levels of fasting blood glucose,fasting insulin,blood urea nitrogen,serum creatinine and urine protein as well as the levels of reduced glutathione(GSH),superoxide dismutase(SOD)and malondialdehyde(MDA)in renal tissues were measured.Additionally,the insulin resistance index was calculated.Hematoxylin-eosin,periodic acid-Schiff,and Masson staining techniques were employed to examine the histopathological alterations in the renal tissue.The expressions of Notch signaling pathway-related proteins in renal tissue were detected by immunohistochemical staining and Western blot methods.RESULTS Compared with model group,the histomorphological of renal tissues in the chsⅣ low-and high-dose groups were significantly improved,with significant decreases in renal histological scores,mesangial expansion index,and glomerulosclerosis scores(P＜0.05);the levels of fasting blood glucose,fasting insulin,blood urea nitrogen,serum creatinine,urine protein and homeostasis model assessment for insulin resistance,as well as MDA content,the expression levels of Notch1,Notch intracellular domain,hairy and enhancer of Split 1 and Delta-like protein 1 in renal tissue were all significantly decreased(P＜0.05).The levels of GSH and SOD in renal tissue were significantly elevated(P＜0.05).Moreover,the improvement in these indicators was significantly more pronounced in the chsⅣ high-dose group compared to the chsⅣ low-dose group(P＜0.05).CONCLUSIONS ChsⅣ can ameliorate renal pathological damage and functional impairment in DN rats.Its underlying mechanisms include restoration of glucose homeostasis and insulin sensitivity,attenuation of renal oxidative stress,and suppression of aberrant Notch signaling pathway activation.关键词
竹节参皂苷Ⅳa/糖尿病肾病/胰岛素抵抗/氧化应激/Notch信号通路Key words
chikusetsu saponin Ⅳa/diabetic nephropathy/insulin resistance/oxidative stress/Notch signaling pathway分类
医药卫生引用本文复制引用
王永丽,陈海,田小芳,王雪春,袁立英,刘丹,李忠发,孟艳芳,杨秀勇..竹节参皂苷Ⅳa对糖尿病肾病大鼠的肾脏保护作用及机制[J].中国药房,2026,37(7):908-913,6.基金项目
贵州省科技计划项目(No.黔科合基础-ZK[2024]一般679) （No.黔科合基础-ZK[2024]一般679）
遵义市科技计划项目(No.遵市科合HZ字[2022]73号,No.遵市科合HZ字[2022]72号) （No.遵市科合HZ字[2022]73号,No.遵市科合HZ字[2022]72号）
